Kv7.4 Antibody (S43/6) - BSA Free

Novus Biologicals | Catalog # NBP2-12899

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S43/6

Format

BSA Free
View all Kv7.4 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 2-77 of human KCNQ4

Localization

Plasma Membrane, Basal Cell Membrane

Specificity

Detects approx 77kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Kv7.4 Antibody (S43/6) - BSA Free

Western Blot: Kv7.4 Antibody (S43/6) [NBP2-12899]

Western Blot: Kv7.4 Antibody (S43/6) [NBP2-12899]

Western Blot: Kv7.4 Antibody (S43/6) [NBP2-12899] - Western Blot analysis of Rat tissue lysate showing detection of Kv7.4 protein using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: Kv7.4 Antibody (S43/6) [NBP2-12899]

Immunocytochemistry/ Immunofluorescence: Kv7.4 Antibody (S43/6) [NBP2-12899]

Immunocytochemistry/Immunofluorescence: Kv7.4 Antibody (S43/6) [NBP2-12899] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kv7.4 Antibody (D) Composite.
Immunohistochemistry: Kv7.4 Antibody (S43/6) [NBP2-12899]

Immunohistochemistry: Kv7.4 Antibody (S43/6) [NBP2-12899]

Immunohistochemistry: Kv7.4 Antibody (S43/6) [NBP2-12899] - Immunohistochemistry analysis using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Immunohistochemistry-Frozen: Kv7.4 Antibody (S43/6) [NBP2-12899]

Immunohistochemistry-Frozen: Kv7.4 Antibody (S43/6) [NBP2-12899]

Immunohistochemistry-Frozen: Kv7.4 Antibody (S43/6) [NBP2-12899] - Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 uL for 5 minutes at RT.

Applications for Kv7.4 Antibody (S43/6) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:1000
Application Notes
1 ug/mL of KCNQ4 Antibody was sufficient for detection of KCNQ4 in 10 ug of COS-1 cell lysate transiently expressing KCNQ4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kv7.4

1 ug/mL was sufficient for detection of KCNQ4 in 10ug of COS-1 cell lysate transiently expressing KCNQ4 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Long Name

Potassium voltage-gated channel subfamily KQT member 4

Alternate Names

KCNQ4, KQT-like 4

Gene Symbol

KCNQ4

UniProt

NP_004691.2 (Human)

Additional Kv7.4 Products

Product Documents for Kv7.4 Antibody (S43/6) - BSA Free

Certificate of Analysis

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Product Specific Notices for Kv7.4 Antibody (S43/6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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