Kynureninase Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-14180

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Kynureninase Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: YAIESQLQLHGLNIEESMRMIKPREGEETLRIEDILEVIEKEGDSIAVILFSGVHFYTGQHFNIPAITKAGQAKGCYVGFDLAHAVGNVELYLHDWGVDFACWCSYKYLNAGAGGIAGAFIHEKHAHTIKPALVGWFGHELSTRFK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Kynureninase Antibody - BSA Free

Western Blot: Kynureninase Antibody [NBP2-14180]

Western Blot: Kynureninase Antibody [NBP2-14180]

Western Blot: Kynureninase Antibody [NBP2-14180] - Analysis in human cell lines A-549 and HEK293 using anti-KYNU antibody. Corresponding KYNU RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: Kynureninase Antibody [NBP2-14180]

Immunocytochemistry/ Immunofluorescence: Kynureninase Antibody [NBP2-14180]

Immunocytochemistry/Immunofluorescence: Kynureninase Antibody [NBP2-14180] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Kynureninase Antibody [NBP2-14180]

Immunohistochemistry-Paraffin: Kynureninase Antibody [NBP2-14180]

Immunohistochemistry-Paraffin: Kynureninase Antibody [NBP2-14180] - Staining in human liver and skeletal muscle tissues using anti-KYNU antibody. Corresponding KYNU RNA-seq data are presented for the same tissues.
Western Blot: Kynureninase Antibody [NBP2-14180]

Western Blot: Kynureninase Antibody [NBP2-14180]

Western Blot: Kynureninase Antibody [NBP2-14180] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-308
Immunohistochemistry-Paraffin: Kynureninase Antibody [NBP2-14180]

Immunohistochemistry-Paraffin: Kynureninase Antibody [NBP2-14180]

Immunohistochemistry-Paraffin: Kynureninase Antibody [NBP2-14180] - Staining of human liver shows high expression.
Immunohistochemistry-Paraffin: Kynureninase Antibody [NBP2-14180]

Immunohistochemistry-Paraffin: Kynureninase Antibody [NBP2-14180]

Immunohistochemistry-Paraffin: Kynureninase Antibody [NBP2-14180] - Staining of human skeletal muscle shows low expression as expected.

Applications for Kynureninase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kynureninase

KYNU, also known as Kynureninase, has a 465 amino acid long isoform that is 52 kDa and a short 307 amino acid that is 35 kDa; its highest expressed levels are found in placenta, liver and lung; is a PLP dependent enzyme that catalyses the cleavage of kynurenine (Kyn) into anthranilic acid (Ant), and It can also act on 3hKyn (to produce 3hAnt) and some other (3-arylcarbonyl)-alanines, and has cysteine-conjugate-beta-lyase activity. This protein is currently being studied for research on the following diseases and disorders: transient cerebral ischemia, vitamin b deficiency, hydroxykynureninuria, pellagra, cerebritis, ischemia, atopic dermatitis, intrahepatic cholangiocarcinoma, essential hypertension, noma, brain injury, dermatitis, psoriasis cholangiocarcinoma, neurologic diseases, interferon, and neuronitis. Interactions with KYNU protein have been shown to involve 40 proteins including GCK, PIN1, NEDD4L, HSPA9, and ETFA in tryptophan catabolism, metabolism, metabolism of amino acids and derivatives, and NAD metabolism pathways.

Alternate Names

KYNU, L-kynurenine hydrolase

Gene Symbol

KYNU

Additional Kynureninase Products

Product Documents for Kynureninase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Kynureninase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Kynurenine Pathway

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Protocols

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