L1CAM Antibody (OTI2A6)

Novus Biologicals | Catalog # NBP2-02149

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2A6
View all L1CAM Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Axon Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for L1CAM Antibody (OTI2A6)

Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149]

Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149]

Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunocytochemistry/ Immunofluorescence: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunocytochemistry/Immunofluorescence: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded pancreas tissue using anti-L1CAMmouse monoclonal antibody.
Flow Cytometry: L1CAM Antibody (OTI2A6) [NBP2-02149]

Flow Cytometry: L1CAM Antibody (OTI2A6) [NBP2-02149]

Flow Cytometry: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of Jurkat cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149]

Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149]

Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded endometrium tissue within the normal limits using anti-L1CAM mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-L1CAMmouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded breast tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded colon tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Kidney tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Ovary tissue using anti-L1CAM mouse monoclonal antibody.
Flow Cytometry: L1CAM Antibody (OTI2A6) [NBP2-02149]

Flow Cytometry: L1CAM Antibody (OTI2A6) [NBP2-02149]

Flow Cytometry: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of Hela cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Immunoprecipitation: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunoprecipitation: L1CAM Antibody (OTI2A6) [NBP2-02149]

Immunoprecipitation: L1CAM Antibody (OTI2A6) [NBP2-02149] - Immunoprecipitation of L1CAM by using TrueMab monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, 500ul of DDK tagged L1CAM overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-L1CAM antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Applications for L1CAM Antibody (OTI2A6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

2-4ug/mg

Western Blot

1:1000

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.97 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: L1CAM

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

Long Name

Cell Adhesion Molecule L1

Alternate Names

CAML1, CD171, HSAS, HSAS1, MASA, MIC5, NCAM-L1, S10, SPG1

Entrez Gene IDs

3897 (Human)

Gene Symbol

L1CAM

Additional L1CAM Products

Product Documents for L1CAM Antibody (OTI2A6)

Certificate of Analysis

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Product Specific Notices for L1CAM Antibody (OTI2A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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