L1CAM Antibody (OTI2A6) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71097

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2A6

Format

Azide and BSA Free
View all L1CAM Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse L1CAM Antibody (OTI2A6) - Azide and BSA Free (NBP2-02149) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for L1CAM Antibody (OTI2A6) - Azide and BSA Free

Western Blot: L1CAM Antibody (OTI2A6)Azide and BSA Free [NBP2-71097]

Western Blot: L1CAM Antibody (OTI2A6)Azide and BSA Free [NBP2-71097]

Western Blot: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.
Immunocytochemistry/ Immunofluorescence: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunocytochemistry/ Immunofluorescence: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunocytochemistry/Immunofluorescence: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM.
Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded pancreas tissue using anti-L1CAMmouse monoclonal antibody.
Flow Cytometry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Flow Cytometry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Flow Cytometry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Analysis of Jurkat cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: L1CAM Antibody (OTI2A6)Azide and BSA Free [NBP2-71097]

Western Blot: L1CAM Antibody (OTI2A6)Azide and BSA Free [NBP2-71097]

Western Blot: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.
Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-L1CAMmouse monoclonal antibody.
Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded breast tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded colon tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded endometrium tissue within the normal limits using anti-L1CAM mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.
Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded Kidney tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded Ovary tissue using anti-L1CAM mouse monoclonal antibody.
Flow Cytometry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Flow Cytometry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Flow Cytometry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Analysis of Hela cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Immunoprecipitation: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunoprecipitation: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097]

Immunoprecipitation: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Immunoprecipitation of L1CAM by using a monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, 500ul of DDK tagged L1CAM overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-L1CAM antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Applications for L1CAM Antibody (OTI2A6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunoprecipitation

2-4ug/mg

Western Blot

1:1000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: L1CAM

The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.

Long Name

Cell Adhesion Molecule L1

Alternate Names

CAML1, CD171, HSAS, HSAS1, MASA, MIC5, NCAM-L1, S10, SPG1

Gene Symbol

L1CAM

Additional L1CAM Products

Product Documents for L1CAM Antibody (OTI2A6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for L1CAM Antibody (OTI2A6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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