Lactate Dehydrogenase A/LDHA Antibody (OTI2D11)

Novus Biologicals | Catalog # NBP1-47822

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D11
View all Lactate Dehydrogenase A/LDHA Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LDHA (NP_005557) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

36.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lactate Dehydrogenase A/LDHA Antibody (OTI2D11)

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lactate Dehydrogenase A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Lactate Dehydrogenase A.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
Immunoprecipitation: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunoprecipitation: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822]

Immunoprecipitation: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Lane 1: Negative Control Lane 2: NBP1-47824 Lane 3: NBP1-47825 Lane 4: NBP1-47823 Lane 5: NBP1-47822
Lactate Dehydrogenase A/LDHA Antibody (OTI2D11)

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822] -

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LDHA monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Applications for Lactate Dehydrogenase A/LDHA Antibody (OTI2D11)

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:1000
Application Notes
WB, ICC/IF reactivity reported in (PMID: 22659615).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase A/LDHA

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

Alternate Names

LDH1, LDHA, LDHM

Entrez Gene IDs

3939 (Human)

Gene Symbol

LDHA

Additional Lactate Dehydrogenase A/LDHA Products

Product Documents for Lactate Dehydrogenase A/LDHA Antibody (OTI2D11)

Certificate of Analysis

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Product Specific Notices for Lactate Dehydrogenase A/LDHA Antibody (OTI2D11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Lactate Dehydrogenase A/LDHA Antibody (OTI2D11)

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