Lactate Dehydrogenase B Antibody (2H6) - Azide and BSA Free

Novus Biologicals | Catalog # H00003945-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Hamster

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2H6

Format

Azide and BSA Free
View all Lactate Dehydrogenase B/LDHB Primary Antibodies »

Product Specifications

Immunogen

LDHB (AAH02362.1, 1 a.a. ~ 334 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSDINQKLKDDEVAQLKKSADTLWDIQKDLKDL

Specificity

LDHB - lactate dehydrogenase B

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Lactate Dehydrogenase B Antibody (2H6) - Azide and BSA Free

Western Blot: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01]

Western Blot: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01]

Western Blot: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01] - LDHB monoclonal antibody (M01), clone 2H6 Analysis of LDHB expression in Hela.
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01]

Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01]

Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01] - Analysis of monoclonal antibody to LDHB on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01] - Analysis of monoclonal antibody to LDHB on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 5 ug/ml.
ELISA: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01]

ELISA: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01]

ELISA: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01] - Detection limit for recombinant GST tagged LDHB is approximately 1ng/ml as a capture antibody.

Applications for Lactate Dehydrogenase B Antibody (2H6) - Azide and BSA Free

Application
Recommended Usage

Simple Western

1:20

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. See Simple Western Antibody Database for Simple Western validation: tested in sorted induced neurons from human; antibody dilution of 1:20; separated by charge; detects a band at 38 kDa

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase B/LDHB

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor (2).

Alternate Names

Epididymis Secretory Protein Li 281, HEL-S-281, LDH-H, LDHB, LDHBD, Renal Carcinoma Antigen NY-REN-46, TRG-5

Entrez Gene IDs

3945 (Human)

Gene Symbol

LDHB

OMIM

150100 (Human)

UniProt

AAH02362 (Human)

Additional Lactate Dehydrogenase B/LDHB Products

Product Documents for Lactate Dehydrogenase B Antibody (2H6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Lactate Dehydrogenase B Antibody (2H6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Lactate Dehydrogenase B Antibody (2H6) - Azide and BSA Free

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Protocols

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FAQs

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