Lactate Dehydrogenase B Antibody

Novus Biologicals | Catalog # NBP1-31945

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Canine (100%), Chimpanzee (100%), Porcine (96%), Rabbit (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Lactate Dehydrogenase B/LDHB Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lactate Dehydrogenase B. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%), Xenopus laevis (81%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lactate Dehydrogenase B Antibody

Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945]

Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945]

Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP1-31945]

Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP1-31945]

Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP1-31945] - Analysis of methanol-fixed HeLa, using antibody at 1:50 dilution.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP1-31945]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP1-31945]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP1-31945] - Mouse liver. LDH-B stained by LDH-B antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945]

Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945]

Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody diluted at 1:500.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP1-31945]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP1-31945]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP1-31945] - Human gastric cancer. LDH-B antibody dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Lactate Dehydrogenase B Antibody

Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945] -

Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945] - Wild-type (WT) and LDH-B knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Lactate Dehydrogenase B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase B/LDHB

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor (2).

Alternate Names

Epididymis Secretory Protein Li 281, HEL-S-281, LDH-H, LDHB, LDHBD, Renal Carcinoma Antigen NY-REN-46, TRG-5

Gene Symbol

LDHB

Additional Lactate Dehydrogenase B/LDHB Products

Product Documents for Lactate Dehydrogenase B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lactate Dehydrogenase B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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