Lactate Dehydrogenase C Antibody
Novus Biologicals | Catalog # NB100-68159
![Western Blot: Lactate Dehydrogenase C Antibody [NB100-68159]](https://resources.rndsystems.com/images/products/Lactate-Dehydrogenase-C-Antibody-Western-Blot-NB100-68159-img0003.jpg "Western Blot: Lactate Dehydrogenase C Antibody [NB100-68159]")
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Key Product Details
Species Reactivity
Validated:
Mouse
Predicted:
Human (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-KLGTDSDKEHWKNIH corresponding to internal region according to NP_002292.1, NP_059144.1.
Epitope
KLGTDSDKEHWKNIH
Specificity
Both variants represent identical product (NP_002292.1 and NP_059144.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Lactate Dehydrogenase C Antibody
Western Blot: Lactate Dehydrogenase C Antibody [NB100-68159]
Western Blot: Lactate Dehydrogenase C Antibody [NB100-68159] - Lactate Dehydrogenase C Antibody [NB100-68159] (0.03ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NB100-68159]](https://resources.rndsystems.com/images/products/Lactate-Dehydrogenase-C-Antibody-Immunohistochemistry-Paraffin-NB100-68159-img0001.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NB100-68159]")
Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NB100-68159]
Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NB100-68159] - (1ug/ml) staining of paraffin embedded Mouse Testis. Data kindly provided by Dr. Erwin Goldberg, Northwestern University, Evanston, IL USA.Applications for Lactate Dehydrogenase C Antibody
Application
Recommended Usage
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
1:10 - 1:500
Peptide ELISA
Detection limit 1:16000
Western Blot
0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 30-35 kDa band observed in mouse testis lysates (calculated MW of 36.6 kDa band according to human NP_002292.1 and 35.9 kDa band according to mouse NP_038608.1). IHC: Strong signal in seminiferous tubules of mouse testis.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Lactate Dehydrogenase C
Alternate Names
Cancer/testis antigen 32, CT32EC 1.1.1.27, EC 1.1.1, lactate dehydrogenase C, lactate dehydrogenase C4, LDH testis subunit, LDH3MGC111073, LDH-C, LDHX, LDH-X, L-lactate dehydrogenase C chain
Gene Symbol
LDHC
UniProt
Additional Lactate Dehydrogenase C Products
Product Documents for Lactate Dehydrogenase C Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Lactate Dehydrogenase C Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Lactate Dehydrogenase C Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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