Lactate Dehydrogenase C Antibody

Novus Biologicals | Catalog # NBP2-19321

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human lactate dehydrogenase C. The exact sequence is proprietary.

Reactivity Notes

Dog (89%), Pig (88%), Bovine (85%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lactate Dehydrogenase C Antibody

Western Blot: Lactate Dehydrogenase C Antibody [NBP2-19321]

Western Blot: Lactate Dehydrogenase C Antibody [NBP2-19321]

Western Blot: Lactate Dehydrogenase C Antibody [NBP2-19321] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP2-19321]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP2-19321]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP2-19321] - Paraffin-embedded ovarian carcinoma. lactate dehydrogenase C antibody dilution: 1:500.
Lactate Dehydrogenase C Antibody

Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase C Antibody [NBP2-19321] -

lactate dehydrogenase C antibody detects lactate dehydrogenase C protein at cytoplasm by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: lactate dehydrogenase C protein stained by lactate dehydrogenase C antibody (NBP2-19321) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for Lactate Dehydrogenase C Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase C

Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region. [provided by RefSeq]

Alternate Names

Cancer/testis antigen 32, CT32EC 1.1.1.27, EC 1.1.1, lactate dehydrogenase C, lactate dehydrogenase C4, LDH testis subunit, LDH3MGC111073, LDH-C, LDHX, LDH-X, L-lactate dehydrogenase C chain

Gene Symbol

LDHC

UniProt

Additional Lactate Dehydrogenase C Products

Product Documents for Lactate Dehydrogenase C Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lactate Dehydrogenase C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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