Lamin A + C Antibody (mab636)

Novus Biologicals | Catalog # NB100-74451

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # mab636
View all Lamin A + C Primary Antibodies »

Product Specifications

Immunogen

Porcine lamin preparation.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 2209722). Mouse reactivity reported in scientific literature (PMID: 11373684).

Localization

Nuclear

Marker

Nuclear Envelope Marker

Specificity

Detects lamin A/C from human, bovine and porcine samples.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Lamin A + C Antibody (mab636)

Western Blot: Lamin A + C Antibody (mab636) [NB100-74451]

Western Blot: Lamin A + C Antibody (mab636) [NB100-74451]

Western Blot: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of various amounts of Human fibroblast whole cell lysate.
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (green) in Human fibroblast cells. Methanol fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C, at a dilution of 1:200 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature.
Western Blot: Lamin A + C Antibody (mab636) [NB100-74451]

Western Blot: Lamin A + C Antibody (mab636) [NB100-74451]

Western Blot: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of 10ug nuclear extract from fractionated mouse tissue lysate.
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (red) in HeLa cells at high magnification.
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) shows staining in Hela Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) shows staining in U251 Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C in HMVEC Cells.
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C in A549 Cells.
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (green) in untreated U2-OS cells (A) or HeLa cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 0.3% BSA for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C, at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.

Applications for Lamin A + C Antibody (mab636)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100

Western Blot

1:100 - 1:2000
Application Notes
WB: Detects an approx. 70 and 65 kDa proteins corresponding to lamin A and C, respectively, from HeLa cell extract.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Tissue culture supernatant

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lamin A + C

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

Alternate Names

CDCD1, CDDC, CMD1A, CMT2B1, dilated 1A (autosomal dominant), EMD2, FPLD, HGPSFPL, lamin A/C, lamin A/C-like 1, lamin-A/C, LDP1, LFP, LGMD1B, limb girdle muscular dystrophy 1B (autosomal dominant), LMN1IDC, LMNC, LMNL1, prelamin-A/C, PRO1,70 kDa lamin, progeria 1 (Hutchinson-Gilford type), renal carcinoma antigen NY-REN-32

Entrez Gene IDs

4000 (Human); 100126859 (Porcine)

Gene Symbol

LMNA

UniProt

Q3ZD69 (Porcine)

Additional Lamin A + C Products

Product Documents for Lamin A + C Antibody (mab636)

Certificate of Analysis

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Product Specific Notices for Lamin A + C Antibody (mab636)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Lamin A + C Antibody (mab636)

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Protocols

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