Lamin A + C Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-19324

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Bovine (98%), Canine (98%), Chimpanzee (100%), Porcine (98%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Lamin A + C Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lamin A + C. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (81%).

Localization

Nucleus

Marker

Nuclear Envelope Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lamin A + C Antibody - Azide Free

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody [NBP2-19324]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody [NBP2-19324]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-19324] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Lamin A + C stained by Lamin A + C antibody diluted at 1:1000. Red: alpha Tubulin, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324]

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324]

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mouse stomach. Lamin A + C stained by Lamin A + C antibody diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324]

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324]

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - C2C12 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324]

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324]

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - RT2 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324]

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324]

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Human breast carcinoma. Lamin A + C antibody diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324]

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324]

Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mahlarvu xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Lamin A + C Antibody

Western Blot: Lamin A + C Antibody [NBP2-19324] -

Western Blot: Lamin A + C Antibody [NBP2-19324] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Lamin A + C Antibody - BSA Free

Western Blot: Lamin A + C Antibody [NBP2-19324] -

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
NBP2-19324 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Lamin A + C Antibody - BSA Free

Immunoprecipitation: Lamin A + C Antibody [NBP2-19324] -

Lamin A + C antibody immunoprecipitates Lamin A + C protein in IP experiments.
IP samples: HeLa whole cell extract
A. 50 ug HeLa whole cell extract
B. Control with 4 ug of preimmune Rabbit IgG
C. Immunoprecipitation of Lamin A + C protein by 4 ug Lamin A + C antibody (NBP2-19324)
7.5 % SDS-PAGE
The immunoprecipitated Lamin A + C protein was detected by Lamin A + C antibody (NBP2-19324) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
Lamin A + C Antibody - BSA Free

Western Blot: Lamin A + C Antibody [NBP2-19324] -

Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (NBP2-19324) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Lamin A + C Antibody - BSA Free

Western Blot: Lamin A + C Antibody [NBP2-19324] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (NBP2-19324) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Lamin A + C Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lamin A + C

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq]

Alternate Names

CDCD1, CDDC, CMD1A, CMT2B1, dilated 1A (autosomal dominant), EMD2, FPLD, HGPSFPL, lamin A/C, lamin A/C-like 1, lamin-A/C, LDP1, LFP, LGMD1B, limb girdle muscular dystrophy 1B (autosomal dominant), LMN1IDC, LMNC, LMNL1, prelamin-A/C, PRO1,70 kDa lamin, progeria 1 (Hutchinson-Gilford type), renal carcinoma antigen NY-REN-32

Gene Symbol

LMNA

Additional Lamin A + C Products

Product Documents for Lamin A + C Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lamin A + C Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Lamin A + C Antibody - Azide Free

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Protocols

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