Lamin B Receptor Antibody (BB2SS3F3) - BSA Free

Novus Biologicals | Catalog # NBP2-59947

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # BB2SS3F3

Format

BSA Free
View all Lamin B Receptor Primary Antibodies »

Product Specifications

Immunogen

GST fused to N-terminal fragment of human Lamin B Receptor (amino acids residues 1-211)

Specificity

Specificity of human, mouse Lamin B Receptor antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Lamin B Receptor Antibody (BB2SS3F3) - BSA Free

Western Blot: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947]

Western Blot: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947]

Western Blot: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] - Analysis of whole cell lysate from human (Hela) and mouse (C2C12, immortalized mouse adult fibroblasts (MAFs), NIE-115) cell lines were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and blotted with BB2SS3F3 antibody. Both primary and secondary antibodies were diluted in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). The predicted molecular weight is 70.7kDA and 71.4kDa for human and mouse LBR respectively. The absence of a band in LBR null MAFs indicated that the antibody specifically recognizes LBR.
Immunocytochemistry/ Immunofluorescence: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947]

Immunocytochemistry/ Immunofluorescence: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947]

Immunocytochemistry/Immunofluorescence: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] - The antibody specifically detects LBR. Wild-type immortalized mouse adult fibroblast (MAFs LBR+/+) (upper panel) and LBR-null MEFs (MAFs LBR-/-) (lower panel) were methanol fixed and immunofluorescently stained with BB2SS3F3 mouse monoclonal antibody. In LBR-null MEFs there is a loss of BB2SS3F3 antibody staining signal indicating the LBR specific signal of the BB2SS3F3 antibody.

Immunohistochemistry-Frozen: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] -

Immunohistochemistry-Frozen: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] - Staining of mouse small intestine. Small intestine from adult C57BL/6 mice was transferred to OCT compound and frozen in liquid nitrogen. Tissue cryosections were fixed in 10% neutral buffered formalin, permeabilized and stained with anti-LBR BB2SS3F3 antibody.
Immunocytochemistry/ Immunofluorescence: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947]

Immunocytochemistry/ Immunofluorescence: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947]

Immunocytochemistry/Immunofluorescence: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] - Confocal images of human (Hela) and mouse (NIE-115) cell lines immunofluorescently stained with BB2SS3F3 antibody. The BB2SS3F3 antibody works on cells fixed with 4% PFA and cold methanol with methanol fixation resulting in brighter signal of the antibody.

Applications for Lamin B Receptor Antibody (BB2SS3F3) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:100 - 1-2000
Application Notes
ICC/IF: fixation with methanol fixation resulting in a brighter signal. Positive Control: C2C12, mouse fibroblast

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lamin B Receptor

Lamins are nuclear membrane proteins that serve to maintain specific cellular functions, such as DNA replication and chromatin organization (1). Lamin B receptor (LBR) is an integral protein of the nuclear envelope inner membrane. It is phosphorylated by CDC2 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures (2). The cleavage of lamins results in nuclear disregulation and cell death (3,4).

Alternate Names

DHCR14B, FLJ43126, Integral nuclear envelope inner membrane protein, lamin B receptor, lamin-B receptor, LMN2R, MGC9041, PHA

Gene Symbol

LBR

Additional Lamin B Receptor Products

Product Documents for Lamin B Receptor Antibody (BB2SS3F3) - BSA Free

Certificate of Analysis

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Product Specific Notices for Lamin B Receptor Antibody (BB2SS3F3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Lamin B Receptor Antibody (BB2SS3F3) - BSA Free

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Protocols

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