Lamin B1 Antibody (SI17-07)

Novus Biologicals | Catalog # NBP2-67405

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SI17-07 expressed in HEK293
View all Lamin B1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Lamin B1 aa 511-560 / 586. (SwissProt: P20700 Human; SwissProt: P14733 Mouse; SwissProt: P70615 Rat)

Localization

Nucleus inner membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lamin B1 Antibody (SI17-07)

Western Blot: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Western Blot: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: Mouse liver tissue lysate, 20 ug/Lane Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of HepG2 cells labeling Lamin B1 with Rabbit anti-Lamin B1 antibody permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lamin B1 antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western Blot: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Western Blot: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Western Blot: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of Lamin B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysateLane 2: Jurkat cell lysate
Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human colon cancer tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human liver tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded mouse colon tissue using anti-Lamin B1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Lamin B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-07) [NBP2-67405]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (SI17-06) [NBP2-67405] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Lamin B1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for Lamin B1 Antibody (SI17-07)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lamin B1

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane.Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

Alternate Names

ADLD, LMN2, LMNB1

Gene Symbol

LMNB1

Additional Lamin B1 Products

Product Documents for Lamin B1 Antibody (SI17-07)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Lamin B1 Antibody (SI17-07)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Epigenetics

Citations for Lamin B1 Antibody (SI17-07)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Lamin B1 Antibody (SI17-07)

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  • Q: I'm looking for human lamin antibodies that might cross react with Drosophila lamins. I've found a few that I'm interested in, one of which comes in a smaller sample size. The other two do not have a smaller size ordering option. I was wondering if it would be possible to get these other antibodies in a smaller size as well, so we can test reactivity with Drosophila lamins before ordering the full-sized product?

    A:

    We have a wide range of antibodies to human Lamin. Unfortunately none of these has yet been tested against Drosophila samples, however you may be interested in our Innovator's Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. We are unable to offer free samples of our antibodies but, as you rightly point out, some are available as smaller, sample size aliquots. If a sample size is not listed for a product I am afraid we are unable to offer a smaller aliquot than those already listed.

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