Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44416

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3112

Format

Azide and BSA Free
View all Laminin alpha 3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q16787, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free

Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Analysis in human stomach and lymph node tissues using NBP3-44416 antibody. Corresponding Laminin alpha 3 RNA-seq data are presented for the same tissues.
Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Staining of human colon shows moderate positivity in basement membrane of glandular cells.
Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Staining of human oral mucosa shows moderate positivity in basement membrane of squamous epithelial cells.
Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Staining of human lymph node shows no positivity in lymphoid cells as expected.
Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Immunohistochemistry-Paraffin: Laminin alpha 3 Antibody [NBP3-44416]

Staining of human stomach shows moderate positivity in basement membrane of glandular cells.
Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free Western Blot: Laminin alpha 3 Antibody [NBP3-44416]

Western Blot: Laminin alpha 3 Antibody [NBP3-44416]

Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.

Applications for Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Laminin alpha 3

Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. The protein encoded by this gene is the alpha-3 subunit of laminin 5, which is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. (provided by RefSeq)

Alternate Names

BM600, BM600 150kD subunit, BM600-150kDa, E170, epiligrin, Epiligrin 170 kDa subunit, epiligrin alpha 3 subunit, Epiligrin subunit alpha, kalinin 165kD subunit, Kalinin subunit alpha, kalinin-165kDa, lama3a, laminin subunit alpha-3, laminin, alpha 3, laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin), laminin-5 alpha 3 chain, Laminin-5 subunit alpha, Laminin-6 subunit alpha, Laminin-7 subunit alpha, LAMNA, LOCS, nicein 150kD subunit, Nicein subunit alpha, nicein-150kDa

Gene Symbol

LAMA3

Additional Laminin alpha 3 Products

Product Documents for Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free and earn rewards!

Have you used Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies