Laminin Antibody (CL2968) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43781
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL2968
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P25391, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Laminin Antibody (CL2968) - Azide and BSA Free
![Laminin Antibody (CL2968) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]](https://resources.rndsystems.com/images/products/nbp3-43781_mouse-laminin-mab-cl2968-azide-and-bsa-free-2822025128215.jpg "Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]")
![Laminin Antibody (CL2968) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]](https://resources.rndsystems.com/images/products/nbp3-43781_mouse-laminin-mab-cl2968-azide-and-bsa-free-282202512324894.jpg "Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]")
Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]
Staining of human gastrointestinal shows no positivity in basement membrane of glandular cells as expected.![Laminin Antibody (CL2968) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]](https://resources.rndsystems.com/images/products/nbp3-43781_mouse-laminin-mab-cl2968-azide-and-bsa-free-28220251353299.jpg "Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]")
Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]
Staining of human pancreas shows no positivity in exocrine glandular cells as expected.![Laminin Antibody (CL2968) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]](https://resources.rndsystems.com/images/products/nbp3-43781_mouse-laminin-mab-cl2968-azide-and-bsa-free-282202513245460.jpg "Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]")
Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]
Staining of human testis shows strong positivity in basement membrane in seminiferous ducts.![Laminin Antibody (CL2968) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]](https://resources.rndsystems.com/images/products/nbp3-43781_mouse-laminin-mab-cl2968-azide-and-bsa-free-282202513415676.jpg "Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]")
Immunohistochemistry-Paraffin: Laminin Antibody [NBP3-43781]
Staining of human tonsil shows no positivity in lymphoid cells as expected.![Laminin Antibody (CL2968) - Azide and BSA Free Western Blot: Laminin Antibody [NBP3-43781]](https://resources.rndsystems.com/images/products/nbp3-43781_mouse-laminin-mab-cl2968-azide-and-bsa-free-28220251335865.jpg "Western Blot: Laminin Antibody [NBP3-43781]")
Western Blot: Laminin Antibody [NBP3-43781]
Analysis in human testis tissue.![Laminin Antibody (CL2968) - Azide and BSA Free Western Blot: Laminin Antibody [NBP3-43781]](https://resources.rndsystems.com/images/products/nbp3-43781_mouse-laminin-mab-cl2968-azide-and-bsa-free-28220251337811.jpg "Western Blot: Laminin Antibody [NBP3-43781]")
Western Blot: Laminin Antibody [NBP3-43781]
Analysis of purified human recombinant Laminin-111, Laminin-121, Laminin-221, Laminin-332, Laminin-411 and Laminin-521.Applications for Laminin Antibody (CL2968) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: Laminin
Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).
References
1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214
2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567
3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y.,... Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5
Alternate Names
LAMA, Laminin A chain, laminin subunit alpha-1, laminin, alpha 1, Laminin-1 subunit alpha, Laminin-3 subunit alpha, PTBHS, S-LAM alpha, S-laminin subunit alpha
Gene Symbol
LAMA1
Additional Laminin Products
Product Documents for Laminin Antibody (CL2968) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Laminin Antibody (CL2968) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Laminin Antibody (CL2968) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review Laminin Antibody (CL2968) - Azide and BSA Free and earn rewards!
Have you used Laminin Antibody (CL2968) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Laminin Antibody (CL2968) - Azide and BSA Free
Showing
1
-
4 of
4 FAQs
Showing All
-
Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?
A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.
-
Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
-
Q: What is the theoretical molecular weight for your Laminin antibodies?
A: The theoretical molecular weight for Laminin antibodies is 337 kDa.
-
Q: What research areas can this product be used in?
A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.
-
Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?
A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.
-
Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
-
Q: What is the theoretical molecular weight for your Laminin antibodies?
A: The theoretical molecular weight for Laminin antibodies is 337 kDa.
-
Q: What research areas can this product be used in?
A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.
-
Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?
A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.
-
Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
-
Q: What is the theoretical molecular weight for your Laminin antibodies?
A: The theoretical molecular weight for Laminin antibodies is 337 kDa.
-
Q: What research areas can this product be used in?
A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.
-
Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?
A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.
-
Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
-
Q: What is the theoretical molecular weight for your Laminin antibodies?
A: The theoretical molecular weight for Laminin antibodies is 337 kDa.
-
Q: What research areas can this product be used in?
A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.
Loading...