Laminin beta 1 Antibody (CL2970) - BSA Free

Novus Biologicals | Catalog # NBP2-42385

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2970

Format

BSA Free
View all Laminin beta 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P07942, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Expected species cross reactivity based on sequence identity: Rat (89%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin beta 1 Antibody (CL2970) - BSA Free

Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Analysis of purified human recombinant Laminin-111, Laminin-211, Laminin-411, Laminin-511, Laminin-521 and Laminin-332.
Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Staining of human lymph node shows absence of immunoreactivity in lymphoid cells (negative control).
Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Analysis in human placenta tissue.
Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Staining of human placenta shows moderate immunoreactivity in basement membrane of trophoblast and in endothelium.
Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Staining of human pancreas shows moderate positivity in basement membrane of exocrine glands.
Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Staining of human endometrium shows immunoreactivity in basement membrane of glandular epithelium.
Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Staining of human stomach shows positivity in basement membrane of glandular epithelium.

Applications for Laminin beta 1 Antibody (CL2970) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin beta 1

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing type IV collagen networks, nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble and bind to other matrix macromolecules, and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Alternate Names

CLM, cutis laxa with marfanoid phenotype, Laminin B1 chain, laminin subunit beta-1, laminin, beta 1, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, MGC142015

Gene Symbol

LAMB1

UniProt

P07942 (Human)

Additional Laminin beta 1 Products

Product Documents for Laminin beta 1 Antibody (CL2970) - BSA Free

Certificate of Analysis

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Product Specific Notices for Laminin beta 1 Antibody (CL2970) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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