Laminin beta 1 Antibody (CL2970) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44411

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2970

Format

Azide and BSA Free
View all Laminin beta 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P07942, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin beta 1 Antibody (CL2970) - Azide and BSA Free

Laminin beta 1 Antibody (CL2970) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 1 Antibody [NBP3-44411]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody [NBP3-44411]

Staining of human endometrium shows immunoreactivity in basement membrane of glandular epithelium.
Laminin beta 1 Antibody (CL2970) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 1 Antibody [NBP3-44411]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody [NBP3-44411]

Staining of human pancreas shows moderate positivity in basement membrane of exocrine glands.
Laminin beta 1 Antibody (CL2970) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 1 Antibody [NBP3-44411]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody [NBP3-44411]

Staining of human lymph node shows absence of immunoreactivity in lymphoid cells (negative control).
Laminin beta 1 Antibody (CL2970) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 1 Antibody [NBP3-44411]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody [NBP3-44411]

Staining of human placenta shows moderate immunoreactivity in basement membrane of trophoblast and in endothelium.
Laminin beta 1 Antibody (CL2970) - Azide and BSA Free Western Blot: Laminin beta 1 Antibody [NBP3-44411]

Western Blot: Laminin beta 1 Antibody [NBP3-44411]

Analysis in human placenta tissue.
Laminin beta 1 Antibody (CL2970) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 1 Antibody [NBP3-44411]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody [NBP3-44411]

Staining of human stomach shows positivity in basement membrane of glandular epithelium.
Laminin beta 1 Antibody (CL2970) - Azide and BSA Free Western Blot: Laminin beta 1 Antibody [NBP3-44411]

Western Blot: Laminin beta 1 Antibody [NBP3-44411]

Analysis of purified human recombinant Laminin-111, Laminin-211, Laminin-411, Laminin-511, Laminin-521 and Laminin-332.

Applications for Laminin beta 1 Antibody (CL2970) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laminin beta 1

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing type IV collagen networks, nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble and bind to other matrix macromolecules, and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Alternate Names

CLM, cutis laxa with marfanoid phenotype, Laminin B1 chain, laminin subunit beta-1, laminin, beta 1, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, MGC142015

Gene Symbol

LAMB1

Additional Laminin beta 1 Products

Product Documents for Laminin beta 1 Antibody (CL2970) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Laminin beta 1 Antibody (CL2970) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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