Laminin beta 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32043

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Laminin beta 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Laminin beta 1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (89%).

Localization

Secreted, extracellular space, extracellular matrix, basement membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

198 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Laminin beta 1 Antibody - BSA Free

Western Blot: Laminin beta 1 Antibody [NBP1-32043]

Western Blot: Laminin beta 1 Antibody [NBP1-32043]

Western Blot: Laminin beta 1 Antibody [NBP1-32043] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Laminin beta 1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody [NBP1-32043]

Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody [NBP1-32043]

Immunocytochemistry/Immunofluorescence: Laminin beta 1 Antibody [NBP1-32043] - Paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: Laminin beta 1 Antibody [NBP1-32043]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody [NBP1-32043]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody [NBP1-32043] - Human colon carcinoma, using Laminin beta 1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Laminin beta 1 Antibody [NBP1-32043]

Western Blot: Laminin beta 1 Antibody [NBP1-32043]

Western Blot: Laminin beta 1 Antibody [NBP1-32043] - Sample (30 ug of whole cell lysate) A: NIH-3T3 5% SDS PAGE; antibody diluted at 1:1000.

Applications for Laminin beta 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Laminin beta 1

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing type IV collagen networks, nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble and bind to other matrix macromolecules, and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Alternate Names

CLM, cutis laxa with marfanoid phenotype, Laminin B1 chain, laminin subunit beta-1, laminin, beta 1, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, MGC142015

Gene Symbol

LAMB1

Additional Laminin beta 1 Products

Product Documents for Laminin beta 1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Laminin beta 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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