Laminin beta 3 Antibody (CL3363) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43800

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3363

Format

Azide and BSA Free
View all Laminin beta 3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q13751, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin beta 3 Antibody (CL3363) - Azide and BSA Free

Laminin beta 3 Antibody (CL3363) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43800]

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43800]

Analysis in human duodenum and pancreas tissues using NBP3-43800 antibody. Corresponding Laminin beta 3 RNA-seq data are presented for the same tissues.
Laminin beta 3 Antibody (CL3363) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43800]

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43800]

Staining of human fallopian tube shows strong positivity in basement membrane in glandular cells.
Laminin beta 3 Antibody (CL3363) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43800]

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43800]

Staining of human duodenum shows moderate positivity in basement membrane in glandular cells.
Laminin beta 3 Antibody (CL3363) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43800]

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43800]

Staining of human prostate shows moderate positivity in basement membrane in glandular cells.
Laminin beta 3 Antibody (CL3363) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43800]

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43800]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Laminin beta 3 Antibody (CL3363) - Azide and BSA Free Western Blot: Laminin beta 3 Antibody [NBP3-43800]

Western Blot: Laminin beta 3 Antibody [NBP3-43800]

Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.

Applications for Laminin beta 3 Antibody (CL3363) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laminin beta 3

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.

Alternate Names

BM600-125kDa, Epiligrin subunit bata, FLJ99565, Kalinin B1 chain, Kalinin subunit beta, kalinin-140kDa, LAM5, Laminin 5, Laminin B1k chain, laminin S B3 chain, laminin subunit beta-3, laminin, beta 3, laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD)), Laminin-5 subunit beta, LAMNB1, Nicein subunit beta, nicein-125kDa

Gene Symbol

LAMB3

Additional Laminin beta 3 Products

Product Documents for Laminin beta 3 Antibody (CL3363) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Laminin beta 3 Antibody (CL3363) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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