Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44400

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2976

Format

Azide and BSA Free
View all Laminin S Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P55268, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free

Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Staining of human colon shows strong positivity in basement membrane of glandular epithelium.
Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Staining of human kidney shows strong positivity in renal glomeruli.
Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Staining of human heart shows strong membranous immunoreactivity in cardiomyocytes.
Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Staining of human cortex shows strong immunoreactivity in endothelial cells.
Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Staining of human lymph node shows absence of immunoreactivity in lymphoid cells (negative control).
Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Staining of human skeletal muscle shows strong membranous immunoreactivity in myocytes.
Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free Western Blot: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Western Blot: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Analysis of purified human recombinant Laminin-421, Laminin-521, Laminin-332 and Laminin-111.
Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free Western Blot: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Western Blot: Laminin S/Laminin beta 2 Antibody [NBP3-44400]

Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Laminin S/Laminin beta 2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Applications for Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laminin S

The Laminins comprise a growing family of disulfide-linked heterotrimers consisting of three genetically distinct polypeptide chains, designated alpha, beta and gamma. A major component of the basal lamina, Laminins play a crucial role in providing a scaffolding upon which tissues are assembled and which serves as a physical barrier separating specialized tissues. During embryogenesis and early development, cells migrate along basement membranes, which are required for the polarization of cells. The beta-2 laminin chain plays a role in melanoma spread, promoting tumor migration along the abluminal surface of vessel, a phenomenon which has been termed extra-vascular migratory metastasis.

Long Name

Laminin, beta 2

Alternate Names

LAMB2, Laminin beta 2

Gene Symbol

LAMB2

Additional Laminin S Products

Product Documents for Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Laminin S/Laminin beta 2 Antibody (CL2976) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

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Protocols

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FAQs

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