LAMP-1/CD107a Antibody (CL3482) - BSA Free

Novus Biologicals | Catalog # NBP2-61619

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3482

Format

BSA Free
View all LAMP-1/CD107a Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P11279, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for LAMP-1/CD107a Antibody (CL3482) - BSA Free

Western Blot: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Western Blot: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Western Blot: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human skeletal muscle shows only very low cytoplasmic immunoreactivity.
Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human kidney shows strong granular cytoplasmic immunoreactivity in renal glomerulus and tubules cells.
Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human fallopian tube shows granular cytoplasmic immunoreactivity in epithelial cells.
Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human small intestine shows granular cytoplasmic immunoreactivity in glandular cells.
LAMP-1/CD107a Antibody (CL3482) - BSA Free

Western Blot: LAMP-1/CD107a Antibody (CL3482) - BSA Free [NBP2-61619] -

LAL protein accumulates in the extra-lysosomal compartment in an in vitro model of NAFLD.Huh7 cells were cultured in high-glucose/high-lipid (HGHL) medium for 8 days. A–C Analysis of LAL localization by confocal microscopy. The lysosomal marker LAMP1 (red) and total LAL protein (individuated by antibody against C-term) (green) were shown (A) and their abundance (B) and colocalization (C) were evaluated. Scale bar 25 μm. D, E Western blot analysis of LAL C-terminal domain, LDHB, Tubulin and LAMP-1 in in the cytosolic fraction (D) and evaluation of LAL content in the cytosolic fraction by calculating LAL to tubulin ratio through densitometric analysis (E). Representative images from one experiment out of three giving similar results are reported. Data are expressed as mean +/- SD (*p < 0.05, **p < 0.01 ***p < 0.001, vs CTR; n = 3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34795230), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for LAMP-1/CD107a Antibody (CL3482) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10000 - 1:20000

Immunohistochemistry-Paraffin

1:10000 - 1:20000

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LAMP-1/CD107a

LAMP-1 (lysosome-associated membrane protein 1), also known as CD107a (cluster of differentiation 107a), is a major component of lysosomal membranes that plays an important role in lysosomal biogenesis, autophagy, and cholesterol metabolism (1). LAMP-1 is a type I transmembrane glycoprotein that is expressed on plasma membranes and the membranes of endosomes, autolysosomes, and lysosomes (1,2). Additionally, LAMP-1/CD107a is a commonly used marker for natural killer (NK) cell degranulation (3). LAMP-1 and another lysosomal-associated membrane protein, LAMP-2, together make up about half of all lysosome membrane proteins (1). Additionally, LAMP-1 has a role in presenting carbohydrate ligands to selectins (2). Human LAMP-1 protein is comprised of 417 amino acids (aa) with a theoretical molecular weight of 44.8 kDa; however, glycosylation can increase the molecular weight upwards of 120 kDa (1, 4). Structurally, LAMP-1 protein contains a large luminal/extracellular domain (29-382 aa), a helical transmembrane domain (383-405 aa), and a short cytoplasmic tail (406-417 aa) (1,2). Additionally, the protein has many N- and O-linked glycosylation sites which helps with stability in the membrane (1,2).

LAMP-1 plays an important role in autophagy-mediated ATP-release during apoptosis where lysosomes containing intracellular ATP migrate to the plasma membrane and, during exocytosis, LAMP-1 is exposed to the cell surface (5). Studies have found that knockdown of LAMP-1 blocks the ATP release from the cell (5). Furthermore, an absence of LAMP-1 and LAMP-2 leads to an accumulation of lysosomal cholesterol (6). Lysosomal membrane dysfunction or defects has also been associated with disease development (6,7). For example, one feature of pancreatitis is autophagy impairment which is caused by lysosomal dysfunction and a corresponding decrease in lysosomal-membrane associated proteins LAMP-1 and LAMP-2 (7).

References

1. Eskelinen E. L. (2006). Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Molecular aspects of medicine, 27(5-6), 495-502. https://doi.org/10.1016/j.mam.2006.08.005

2. Cheng, X. T., Xie, Y. X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z. H. (2018). Revisiting LAMP1 as a marker for degradative autophagy-lysosomal organelles in the nervous system. Autophagy, 14(8), 1472-1474. https://doi.org/10.1080/15548627.2018.1482147

3. Krzewski, K., & Coligan, J. E. (2012). Human NK cell lytic granules and regulation of their exocytosis. Frontiers in immunology, 3, 335. https://doi.org/10.3389/fimmu.2012.00335

4. Uniprot (P11279)

5. Wang, Y., Martins, I., Ma, Y., Kepp, O., Galluzzi, L., & Kroemer, G. (2013). Autophagy-dependent ATP release from dying cells via lysosomal exocytosis. Autophagy, 9(10), 1624-1625. https://doi.org/10.4161/auto.25873

6. Schwake, M., Schr0der, B., & Saftig, P. (2013). Lysosomal membrane proteins and their central role in physiology. Traffic (Copenhagen, Denmark), 14(7), 739-748. https://doi.org/10.1111/tra.12056

7. Gukovsky, I., Pandol, S. J., Mareninova, O. A., Shalbueva, N., Jia, W., & Gukovskaya, A. S. (2012). Impaired autophagy and organellar dysfunction in pancreatitis. Journal of gastroenterology and hepatology, 27 Suppl 2(Suppl 2), 27-32. https://doi.org/10.1111/j.1440-1746.2011.07004.x

Long Name

Lysosome-associated Membrane Glycoprotein 1

Alternate Names

CD107a, LAMP1

Gene Symbol

LAMP1

Additional LAMP-1/CD107a Products

Product Documents for LAMP-1/CD107a Antibody (CL3482) - BSA Free

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Product Specific Notices for LAMP-1/CD107a Antibody (CL3482) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LAMP-1/CD107a Antibody (CL3482) - BSA Free

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Protocols

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