LAMTOR1 Antibody

Novus Biologicals | Catalog # NBP1-71689

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all LAMTOR1 Primary Antibodies »

Product Specifications

Immunogen

A genomic peptide made to a C-terminal region of the human c11orf59/LAMTOR1 protein (within residues 50-161). [Swiss-Prot Q6IAA8]

Reactivity Notes

Immunogen has 98% identity to bovine.

Localization

Late endosome membrane; Lipid-anchor; Cytoplasmic side. Lysosome membrane; Lipid-anchor; Cytoplasmic side. Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LAMTOR1 Antibody

Western Blot: LAMTOR1 Antibody [NBP1-71689]

Western Blot: LAMTOR1 Antibody [NBP1-71689]

Western Blot: c11orf59/LAMTOR1 Antibody [NBP1-71689] - Analysis of c11orf59 in human liver protein.
Immunocytochemistry/ Immunofluorescence: LAMTOR1 Antibody [NBP1-71689]

Immunocytochemistry/ Immunofluorescence: LAMTOR1 Antibody [NBP1-71689]

Immunocytochemistry/Immunofluorescence: c11orf59/LAMTOR1 Antibody [NBP1-71689] - C11orf59 antibody was tested at 1:50 in HepG2 cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).
Immunohistochemistry: LAMTOR1 Antibody [NBP1-71689]

Immunohistochemistry: LAMTOR1 Antibody [NBP1-71689]

Immunohistochemistry: c11orf59/LAMTOR1 Antibody [NBP1-71689] - Analysis of C11orf59 in mouse pancreas.

Applications for LAMTOR1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:25-1:100

Immunohistochemistry

1:25-1:100

Immunohistochemistry-Paraffin

1:25-1:100

Western Blot

1:1000
Application Notes
This c11orf59 antibody is useful for IHC-P, ICC/IF and Western blot, where a band is seen ~22 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-71689 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 0.1% BSA, and 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LAMTOR1

c11orf59/LAMTOR1 (ragulator complex protein LAMTOR1 or PDRO), a part of Ragulator complex which contains LAMTOR1-5, is a relatively novel lapidated protein adaptor of the lysosomal membrane which senses and integrates a diverse array of cellular signals, with mTOR kinase activity within the complex being influenced by a variety of nutrients e.g. amino acids, glucose etc., oxygen status, cellular energy levels, and many secreted growth factors such as cytokines and hormones, including insulin. Ragulator is activated by amino acids through a mechanism involving lysosomal V-ATPase, and it functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Ragulator (activated) and Rag GTPases function as a scaffold that recruits mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to membranes and is also required for late endosomes/lysosomes biogenesis. It regulates both the recycling of receptors through endosomes and MAPK signaling pathway through recruitment of some of its components to late endosomes. It implicates in cholesterol homeostasis for regulating LDL uptake and cholesterol release from late endosomes/lysosomes, and may play a role in RHOA activation. LAMTOR1 deregulation is observed in certain pathologies including cancer, diabetes and neurodegenerative diseases.

Alternate Names

c11orf59, chromosome 11 open reading frame 59, FLJ20625, LAMTOR1, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1, late endosomal/lysosomal adaptor, MAPK and MTOR activator 1, Lipid raft adaptor protein p18, p18, p27kip1 releasing factor from RhoA, p27Kip1-releasing factor from RhoA, p27RF-RhoC11orf59, PDRO, PDROPdro, Protein associated with DRMs and endosomes, ragulator complex protein PDRO, Ragulator1, RhoA activator C11orf59

Entrez Gene IDs

55004 (Human); 66508 (Mouse)

Gene Symbol

LAMTOR1

UniProt

Q6IAA8 (Human), Q9CQ22 (Mouse)

Additional LAMTOR1 Products

Product Documents for LAMTOR1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LAMTOR1 Antibody

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LAMTOR1 Antibody

Customer Reviews for LAMTOR1 Antibody (1)

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  • c11orf59/LAMTOR1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MEF whole cell lysate
    Species: Mouse
    Verified Customer | Posted 03/26/2018
    Lysis buffer: 2x Laemmli buffer 20 µg protein /well Ab dilution: 1:1000 in milk-PBST
    LAMTOR1 Antibody NBP1-71689

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Protocols

View specific protocols for LAMTOR1 Antibody (NBP1-71689):

Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures."

LAMTOR1 Antibody:
Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

LAMTOR1 Antibody:
Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.

*Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

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FAQs

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