Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Equine

Applications

Validated:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Product Image

Cited:

IF/IHC

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 929F3.01
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Product Specifications

Immunogen

Langerin/CD207 was used as immunogen.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 488]

Product Image: Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 488] [DDX0362A488-100] - Vial of Alexa Fluor 488 conjugated antibody. Alexa Fluor 488 is optimally excited at 490 nm by the Blue laser (488 nm) and has an emission maximum of 525 nm.

Applications for Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 488]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000
Application Notes
Permeabilization is recommended for flow cytometry.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: Langerin/CD207

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

Alternate Names

CD207

Gene Symbol

CD207

Additional Langerin/CD207 Products

Product Documents for Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 488]

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 488]

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Protocols

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail