Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Equine

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Product Image

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 929F3.01
View all Langerin/CD207 Primary Antibodies »

Product Specifications

Immunogen

Langerin/CD207 was used as immunogen.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 647]

Product Image: Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 647] [DDX0362A647-100] - Vial of Alexa Fluor 647 conjugated antibody. Alexa Fluor 647 is optimally excited at 653 nm by the Red laser (633 or 640 nm) and has an emission maximum of 669 nm.

Applications for Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 647]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000
Application Notes
Permeabilization is recommended for flow cytometry.

Reviewed Applications

Read 1 review rated 3 using DDX0362A647-100 in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: Langerin/CD207

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

Alternate Names

CD207, DDX0362A647

Gene Symbol

CD207

Additional Langerin/CD207 Products

Product Documents for Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 647]

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 647]

Customer Reviews for Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 647] (1)

3 out of 5
1 Customer Rating
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Customer Images

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  • Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 647]
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Monocyte-derived dendritic cells
    Species: Human
    Verified Customer | Posted 08/13/2024
    Detection of human CD207 on monocyte-derived dendritic cells. The cells were treated with 10 μg/ml of the CD207-AF647 antibody (blue) or isotype rat IgG2a AF647 control antibody (red)
    Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 647] DDX0362A647-100

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Protocols

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail