Key Product Details

Species Reactivity

Validated:

Human, Canine

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Binding Activity

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # DCGM4/122D5

Format

Azide and BSA Free
View all Langerin/CD207 Primary Antibodies »

Product Specifications

Immunogen

Langerin/CD207 was used as immunogen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Langerin/CD207 Antibody (DCGM4/122D5) - Azide and BSA Free

Western Blot: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]

Western Blot: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]

Western Blot: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Western-blot DCGM4 1: non-reduced 2: reduced
Immunohistochemistry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]

Immunohistochemistry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]

Immunohistochemistry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - lung tumor tissue section
Flow Cytometry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]
Immunohistochemistry-Frozen: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]
Immunohistochemistry-Frozen: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]
Immunohistochemistry-Frozen: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]

Immunohistochemistry-Frozen: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]

Immunohistochemistry-Frozen: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Analysis of Alexa Fluor (R) 546 conjugate of DDX0363P-100. Staining of human skin frozen section.
Immunohistochemistry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]

Immunohistochemistry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]

Immunohistochemistry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - CHO control
Immunohistochemistry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]

Immunohistochemistry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]

Immunohistochemistry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Langerin-transfected CHO
Langerin/CD207 Antibody (DCGM4/122D5)

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] -

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Langerin co-localizes with caveolin-1 in steady state. Confocal scanning laser microscopic analyses for THP-langerin cell line (A), MUTZ3-LC cell line (MUTZ-LCs, B) & human LCs (C) for langerin & caveolin-1 in steady-state condition in permeablized cells; bars represent 10 μm (A, B, C). Langerin & caveolin-1 were immuno-precipitated from LC cell lysates & immuno-blotted with antibodies against caveolin-1 or langerin (D). These data are representative of at least 3 donors or 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25551286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Langerin/CD207 Antibody (DCGM4/122D5)

Western Blot: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] -

Western Blot: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Langerin co-localizes with caveolin-1 in steady state. Confocal scanning laser microscopic analyses for THP-langerin cell line (A), MUTZ3-LC cell line (MUTZ-LCs, B) & human LCs (C) for langerin & caveolin-1 in steady-state condition in permeablized cells; bars represent 10 μm (A, B, C). Langerin & caveolin-1 were immuno-precipitated from LC cell lysates & immuno-blotted with antibodies against caveolin-1 or langerin (D). These data are representative of at least 3 donors or 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25551286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Langerin/CD207 Antibody (DCGM4/122D5)

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] -

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Langerin co-localizes with caveolin-1 in steady state. Confocal scanning laser microscopic analyses for THP-langerin cell line (A), MUTZ3-LC cell line (MUTZ-LCs, B) & human LCs (C) for langerin & caveolin-1 in steady-state condition in permeablized cells; bars represent 10 μm (A, B, C). Langerin & caveolin-1 were immuno-precipitated from LC cell lysates & immuno-blotted with antibodies against caveolin-1 or langerin (D). These data are representative of at least 3 donors or 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25551286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Langerin/CD207 Antibody (DCGM4/122D5)

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] -

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Langerin co-localizes with caveolin-1 in steady state. Confocal scanning laser microscopic analyses for THP-langerin cell line (A), MUTZ3-LC cell line (MUTZ-LCs, B) & human LCs (C) for langerin & caveolin-1 in steady-state condition in permeablized cells; bars represent 10 μm (A, B, C). Langerin & caveolin-1 were immuno-precipitated from LC cell lysates & immuno-blotted with antibodies against caveolin-1 or langerin (D). These data are representative of at least 3 donors or 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25551286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Langerin/CD207 Antibody (DCGM4/122D5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30223113).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

Tris-NaCl (pH 8.0)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Langerin/CD207

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

Alternate Names

CD207

Gene Symbol

CD207

Additional Langerin/CD207 Products

Product Documents for Langerin/CD207 Antibody (DCGM4/122D5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Langerin/CD207 Antibody (DCGM4/122D5) - Azide and BSA Free

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Langerin/CD207 Antibody (DCGM4/122D5) - Azide and BSA Free

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Protocols

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail