LAPTM4B Antibody (CL5289)
Novus Biologicals | Catalog # NBP3-52052
Recombinant Monoclonal Antibody
![LAPTM4B Antibody (CL5289) Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]](https://resources.rndsystems.com/images/products/nbp3-52052_mouse-laptm4b-mab-cl5289-immunohistochemistry-273202615275523.jpg "Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # CL5289
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Product Specifications
Immunogen
This antibody was developed using a synthetic peptide derived from Q86VI4, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for LAPTM4B Antibody (CL5289)
Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]
Staining of human heart muscle shows moderate granular cytoplasmic positivity in cardiomyocytes.![LAPTM4B Antibody (CL5289) Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]](https://resources.rndsystems.com/images/products/nbp3-52052_mouse-laptm4b-mab-cl5289-immunohistochemistry-27320261523311.jpg "Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]")
Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]
Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.![LAPTM4B Antibody (CL5289) Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]](https://resources.rndsystems.com/images/products/nbp3-52052_mouse-laptm4b-mab-cl5289-immunohistochemistry-2732026152694.jpg "Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]")
Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]
Staining of human cerebral cortex shows moderate to strong granular cytoplasmic positivity in neurons.![LAPTM4B Antibody (CL5289) Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]](https://resources.rndsystems.com/images/products/nbp3-52052_mouse-laptm4b-mab-cl5289-immunohistochemistry-2732026151969.jpg "Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]")
Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]
Analysis in human heart muscle and tonsil tissues. Corresponding LAPTM4B RNA-seq data are presented for the same tissues.![LAPTM4B Antibody (CL5289) Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]](https://resources.rndsystems.com/images/products/nbp3-52052_mouse-laptm4b-mab-cl5289-immunohistochemistry-273202615211911.jpg "Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]")
Immunohistochemistry: LAPTM4B Antibody (CL5289) [NBP3-52052]
Staining of human tonsil shows no positivity in non-germinal center cells as expected.![LAPTM4B Antibody (CL5289) Western Blot: LAPTM4B Antibody (CL5289) [NBP3-52052]](https://resources.rndsystems.com/images/products/nbp3-52052_mouse-laptm4b-mab-cl5289-western-blot-273202615202415.jpg "Western Blot: LAPTM4B Antibody (CL5289) [NBP3-52052]")
Western Blot: LAPTM4B Antibody (CL5289) [NBP3-52052]
Analysis in human cell line HEL.
Immunocytochemistry/ Immunofluorescence: LAPTM4B Antibody (CL5289) [NBP3-52052]
Staining of A-431 cells showing specific staining in vesicles and the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).Applications for LAPTM4B Antibody (CL5289)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LAPTM4B
Alternate Names
LAPTM4beta, LC27, lysosomal associated protein transmembrane 4 beta, lysosomal protein transmembrane 4 beta, lysosomal-associated transmembrane protein 4B, Lysosome-associated transmembrane protein 4-beta
Gene Symbol
LAPTM4B
Additional LAPTM4B Products
Product Documents for LAPTM4B Antibody (CL5289)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LAPTM4B Antibody (CL5289)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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