Latexin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86079

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MEIPPTNYPASRAALVAQNYINYQQGTPHRVFEVQKVKQASMEDIPGRGHKYHLKFAVEEIIQKQVKVNCTAEVLYPSTGQETAPEVNFTFEGETGKNPDEEDNTF

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23364479)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Latexin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Latexin Antibody [NBP1-86079]

Immunocytochemistry/ Immunofluorescence: Latexin Antibody [NBP1-86079]

Immunocytochemistry/Immunofluorescence: Latexin Antibody [NBP1-86079] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.
Latexin Antibody - BSA Free Immunohistochemistry-Paraffin: Latexin Antibody - BSA Free [NBP1-86079]

Immunohistochemistry-Paraffin: Latexin Antibody - BSA Free [NBP1-86079]

Staining of human prostate shows strong cytoplasmic and nuclear positivity in glandular cells.
Latexin Antibody - BSA Free Western Blot: Latexin Antibody - BSA Free [NBP1-86079]

Western Blot: Latexin Antibody - BSA Free [NBP1-86079]

Analysis in human cell line MCF-7.
Latexin Antibody - BSA Free Western Blot: Latexin Antibody - BSA Free [NBP1-86079]

Western Blot: Latexin Antibody - BSA Free [NBP1-86079]

Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LXN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Latexin Antibody - BSA Free Western Blot: Latexin Antibody - BSA Free [NBP1-86079]

Western Blot: Latexin Antibody - BSA Free [NBP1-86079]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for Latexin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Latexin

Latexin, a protein possessing inhibitory activity against rat carboxypeptidase A1 (CPA1) and CPA2, is expressed in a neuronal subset in the cerebral cortex and cells in other neural and non-neural tissues of rat. Although latexin also inhibits mast-cell CPA (MCCPA), the expression of latexin in rat mast cells has not previously been confirmed.

Alternate Names

ECI, LXN, TCI

Gene Symbol

LXN

Additional Latexin Products

Product Documents for Latexin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Latexin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Latexin Antibody - BSA Free

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Protocols

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FAQs

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