LDB1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31362

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (97%), Porcine (98%), Rhesus Macaque (100%), Xenopus (97%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LDB1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human LDB1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (97%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LDB1 Antibody - BSA Free

Knockout Validated: LDB1 Antibody [NBP1-31362]

Western Blot: LDB1 Antibody [NBP1-31362]

Western Blot: LDB1 Antibody [NBP1-31362] - Wild-type (WT) and LDB1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: LDB1 Antibody [NBP1-31362]

Immunocytochemistry/ Immunofluorescence: LDB1 Antibody [NBP1-31362]

Immunocytochemistry/Immunofluorescence: LDB1 Antibody [NBP1-31362] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LDB1 protein stained by LDB1 antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362]

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362]

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - mouse muscle.
Western Blot: LDB1 Antibody [NBP1-31362]

Western Blot: LDB1 Antibody [NBP1-31362]

Western Blot: LDB1 Antibody [NBP1-31362] - Sample: 1000 ug 293T whole cell lysate/extract A. 20 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgGC. Immunoprecipitation of LDB1 protein by 2. 5 ug of LDB1 antibody diluted at 1:1000.
Western Blot: LDB1 Antibody [NBP1-31362]

Western Blot: LDB1 Antibody [NBP1-31362]

Western Blot: LDB1 Antibody [NBP1-31362] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362]

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362]

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - Paraffin-embedded U87 Xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362]

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362]

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - rat middle brain.
LDB1 Antibody

Western Blot: LDB1 Antibody [NBP1-31362] -

Western Blot: LDB1 Antibody [NBP1-31362] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody [N2C3] (NBP1-31362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
LDB1 Antibody - BSA Free

Western Blot: LDB1 Antibody [NBP1-31362] -

IMR32 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody [N2C3] (NBP1-31362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
LDB1 Antibody - BSA Free

Western Blot: LDB1 Antibody [NBP1-31362] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody [N2C3] (NBP1-31362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for LDB1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LDB1

LDB1 binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neuron

Alternate Names

carboxy terminal LIM domain protein 2, Carboxyl-terminal LIM domain-binding protein 2, CLIM-2, CLIM2hLdb1, LDB-1, LIM domain binding 1, LIM domain-binding factor-1, LIM domain-binding protein 1, NLILIM domain-binding factor CLIM2, Nuclear LIM interactor

Gene Symbol

LDB1

Additional LDB1 Products

Product Documents for LDB1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for LDB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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