LDB3 Antibody

Novus Biologicals | Catalog # NB100-2445

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LDB3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KSQNKPEDEADE corresponding to internal region according to NP_009009.1, NP_001073583.1, NP_001073584.1, NP_001073585.1.

Epitope

C-KSQNKPEDEADE

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LDB3 Antibody

Western Blot: LDB3 Antibody [NB100-2445]

Western Blot: LDB3 Antibody [NB100-2445]

Western Blot: LDB3 Antibody [NB100-2445] - Staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LDB3 Antibody

Immunohistochemistry-Paraffin: LDB3 Antibody [NB100-2445] -

Immunohistochemistry-Paraffin: LDB3 Antibody [NB100-2445] - Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.
LDB3 Antibody

Immunohistochemistry-Paraffin: LDB3 Antibody [NB100-2445] -

Immunohistochemistry-Paraffin: LDB3 Antibody [NB100-2445] - (6ug/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for LDB3 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

6-7 ug/ml

Peptide ELISA

Detection limit 1:1000

Western Blot

0.1 - 1 ug/ml
Application Notes
WB: WB experiments gave bands at approx. 90 kDa band and 35 kDa band in human skeletal muscle lysate after 1 ug/ml antibody staining. These bands correspond to earlier findings are reported in scientific literature with different antibodies (PMID: 12499364). This protein has a calculated MW of 77.1 kDa band according to NP_009009.1.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LDB3

Gene ID(s): 11155 (human)

Alternate Names

CMD1C, CYPHER, KIAA01613, KIAA0613FLJ35865, LDB3Z1, LDB3Z4, LIM domain binding 3, LIM domain-binding protein 3, ORACLE, PDLIM6, PDZ and LIM domain 6, Protein cypher, ZASPLVNC3, Z-band alternatively spliced PDZ-motif protein

Entrez Gene IDs

11155 (Human)

Gene Symbol

LDB3

UniProt

NP_009009.1 (Human)

Additional LDB3 Products

Product Documents for LDB3 Antibody

Certificate of Analysis

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Product Specific Notices for LDB3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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