LDLR Antibody (1B10H10) - BSA Free

Novus Biologicals | Catalog # NBP2-52502

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B10H10

Format

BSA Free
View all LDLR Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human LDLR (AA: 22-150) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

95.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LDLR Antibody (1B10H10) - BSA Free

Western Blot: LDLR Antibody (1B10H10)BSA Free [NBP2-52502]

Western Blot: LDLR Antibody (1B10H10)BSA Free [NBP2-52502]

Western Blot: LDL R Antibody (1B10H10) [NBP2-52502] - Western blot analysis using LDLR mAb against human LDLR (AA: 22-150) recombinant protein. (Expected MW is 40.1 kDa)
Immunohistochemistry: LDLR Antibody (1B10H10) - BSA Free [NBP2-52502]

Immunohistochemistry: LDLR Antibody (1B10H10) - BSA Free [NBP2-52502]

Immunohistochemistry: LDL R Antibody (1B10H10) [NBP2-52502] - Analysis of paraffin-embedded ovarian cancertissues using LDLR mouse mAb with DAB staining.
Flow Cytometry: LDLR Antibody (1B10H10) - BSA Free [NBP2-52502]

Flow Cytometry: LDLR Antibody (1B10H10) - BSA Free [NBP2-52502]

Flow Cytometry: LDL R Antibody (1B10H10) [NBP2-52502] - Analysis of Hela cells using LDLR mouse mAb (green) and negative control (red).
Western Blot: LDLR Antibody (1B10H10)BSA Free [NBP2-52502]

Western Blot: LDLR Antibody (1B10H10)BSA Free [NBP2-52502]

Western Blot: LDL R Antibody (1B10H10) [NBP2-52502] - Analysis using LDLR mAb against HEK293 (1) and LDLR (AA: 22-150)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: LDLR Antibody (1B10H10) - BSA Free [NBP2-52502]

ELISA: LDLR Antibody (1B10H10) - BSA Free [NBP2-52502]

ELISA: LDLR Antibody (1B10H10) [NBP2-52502] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for LDLR Antibody (1B10H10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LDLR

The low density lipoprotein (LDL) receptor system coordinates the metabolism of cholesterol, an essential component of the plasma membrane of all mammalian cells. Study of this system has led to an enhanced understanding of the cellular basis of cholesterol homeostasis. It has also brought into focus an important mechanism of metabolic regulation--the process of receptor-mediated endocytosis. Data suggest that the juxtamembranous region of the cytoplasmic domain participates in protein:protein interactions that allow the low density lipoprotein receptor to cluster in coated pits. It has been shown that the family of LDL receptors may serve as viral receptors. Endocytosis of the Flaviviridae viruses, hepatitis C virus, GB virus C/hepatitis G virus, and bovine viral diarrheal virus (BVDV) were shown to be mediated by LDL receptors on cultured cells.

Long Name

Low Density Lipoprotein Receptor

Alternate Names

LDL R

Gene Symbol

LDLR

Additional LDLR Products

Product Documents for LDLR Antibody (1B10H10) - BSA Free

Certificate of Analysis

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Product Specific Notices for LDLR Antibody (1B10H10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

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Protocols

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FAQs

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Associated Pathways

A-beta Pathways: Uptake & Degradation A-beta Pathways: Uptake & Degradation Thumbnail