LEF1 Antibody (REMB6) - BSA Free

Novus Biologicals | Catalog # NBP2-66597

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # REMB6

Format

BSA Free
View all LEF1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for the anti-LEF1 antibody was a full length protein (Uniprot: Q9UJU2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse LEF1 Antibody (REMB6) - BSA Free (NBP2-66597) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LEF1 Antibody (REMB6) - BSA Free

LEF1 Antibody (REMB6)

Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (REMB6) [NBP2-66597] -

Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (REMB6) [NBP2-66597] - Figure 1. Immunofluorescence staining of Cos-1 cells transfected with (top to bottom) full length hLEF1, a LEF1 mutant missing the nuclear localization signal, TCF-4 and TCF -1. Cells were stained with Novus Biologicals' LEF1 antibody, clone REMB6 cat. # T105M and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF/TCF/HMG, clone REMB6 recognizes an epitope in the highly conserved HMG DNA binding domain.

Applications for LEF1 Antibody (REMB6) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1 ug/mL

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1 ug/ml

Western Blot

5-10 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4 °C.

Background: LEF1

Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kD on SDSPAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms. Clone REMB6 recognizes the High Mobility Group DNA domain of LEF-1.

Long Name

Lymphoid Enhancer-binding Factor 1

Alternate Names

TCF10, TCF1ALPHA, TCF7L3

Gene Symbol

LEF1

UniProt

P24821 (Human)

Additional LEF1 Products

Product Documents for LEF1 Antibody (REMB6) - BSA Free

Certificate of Analysis

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Product Specific Notices for LEF1 Antibody (REMB6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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