LEF1 Antibody

Novus Biologicals | Catalog # NB100-41374

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LEF1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QHEQRKEQEPKRPH corresponding to internal region according to NP_057353.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat LEF1 Antibody (NB100-41374) is a polyclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-LEF1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LEF1 Antibody

Western Blot: LEF1 Antibody [NB100-41374]

Western Blot: LEF1 Antibody [NB100-41374]

Western Blot: LEF1 Antibody [NB100-41374] - Staining of K562 nuclear cell lysate (A) + peptide (B). Antibody at 2 ug/mL. 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: LEF1 Antibody [NB100-41374]

Immunocytochemistry/ Immunofluorescence: LEF1 Antibody [NB100-41374]

Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NB100-41374] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: LEF1 Antibody [NB100-41374]

Flow Cytometry: LEF1 Antibody [NB100-41374]

Flow Cytometry: LEF1 Antibody [NB100-41374] - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: LEF1 Antibody [NB100-41374]

Immunocytochemistry/ Immunofluorescence: LEF1 Antibody [NB100-41374]

Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NB100-41374] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for LEF1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/mL
Application Notes
Approx 50-55 kDa band observed in nuclear lysates of cell line K562 (calculated MW of 44.2 kDa according to NP_057353.1). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunizing peptide.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LEF1

Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).

Long Name

Lymphoid Enhancer-binding Factor 1

Alternate Names

TCF10, TCF1ALPHA, TCF7L3

Entrez Gene IDs

51176 (Human); 16842 (Mouse); 161452 (Rat)

Gene Symbol

LEF1

UniProt

NP_057353.1 (Human)

Additional LEF1 Products

Product Documents for LEF1 Antibody

Certificate of Analysis

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Product Specific Notices for LEF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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