LEF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86960

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LEF1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SDVNSKQGMSRHPPAPDIPTFYPLSPGGVGQITPPLGWQGQPVYPITGGFRQPYPSSLSVDTSMSRFSHHMIPGPPGPHTTGIPHPAIVTPQVKQEHPHTDSDLMHVKPQHEQRKEQEPKRPHIKKPLNAFMLY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit LEF1 Antibody - BSA Free (NBP1-86960) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-LEF1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LEF1 Antibody - BSA Free

Western Blot: LEF1 Antibody [NBP1-86960]

Western Blot: LEF1 Antibody [NBP1-86960]

Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in human cell lines SK-MEL-30 and A-549 using anti-LEF1 antibody. Corresponding LEF1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
Western Blot: LEF1 Antibody [NBP1-86960]

Western Blot: LEF1 Antibody [NBP1-86960]

Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in control (vector only transfected HEK293T lysate) and LEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: LEF1 Antibody [NBP1-86960]

Immunocytochemistry/ Immunofluorescence: LEF1 Antibody [NBP1-86960]

Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NBP1-86960] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960]

Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960]

Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining of human oral mucosa shows moderate nuclear positivity in subset of squamous epithelial cells.
Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960]

Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960]

Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining of human lymph node shows moderate to strong nuclear positivity in non - germinal center cells.
LEF1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: LEF1 Antibody - BSA Free [NBP1-86960]

Chromatin Immunoprecipitation-exo-Seq: LEF1 Antibody - BSA Free [NBP1-86960]

ChIP-Exo-Seq composite graph for Anti-LEF1 (NBP1-86960) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for LEF1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LEF1

Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM]

Long Name

Lymphoid Enhancer-binding Factor 1

Alternate Names

TCF10, TCF1ALPHA, TCF7L3

Gene Symbol

LEF1

Additional LEF1 Products

Product Documents for LEF1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LEF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LEF1 Antibody - BSA Free

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Protocols

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FAQs

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