Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI6C10
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Product Specifications
Immunogen
Full length human recombinant protein of human LEMD3 (NP_055134) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for LEMD3 Antibody (OTI6C10)
![Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211]](https://resources.rndsystems.com/images/products/LEMD3-Antibody-OTI6C10-Western-Blot-NBP2-02211-img0007.jpg "Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211]")
Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211]
Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LEMD3 monoclonal antibody.![Immunocytochemistry/ Immunofluorescence: LEMD3 Antibody (OTI6C10) [NBP2-02211]](https://resources.rndsystems.com/images/products/LEMD3-Antibody-OTI6C10-Immunocytochemistry-Immunofluorescence-NBP2-02211-img0006.jpg "Immunocytochemistry/ Immunofluorescence: LEMD3 Antibody (OTI6C10) [NBP2-02211]")
Immunocytochemistry/ Immunofluorescence: LEMD3 Antibody (OTI6C10) [NBP2-02211]
Immunocytochemistry/Immunofluorescence: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LEMD3.![Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211]](https://resources.rndsystems.com/images/products/LEMD3-Antibody-OTI6C10-Immunohistochemistry-Paraffin-NBP2-02211-img0005.jpg "Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211]")
Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211]
Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Staining of paraffin-embedded liver tissue using anti-LEMD3mouse monoclonal antibody.![Flow Cytometry: LEMD3 Antibody (OTI6C10) [NBP2-02211]](https://resources.rndsystems.com/images/products/LEMD3-Antibody-OTI6C10-Flow-Cytometry-NBP2-02211-img0002.jpg "Flow Cytometry: LEMD3 Antibody (OTI6C10) [NBP2-02211]")
Flow Cytometry: LEMD3 Antibody (OTI6C10) [NBP2-02211]
Flow Cytometry: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Analysis of Jurkat cells, using anti-LEMD3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).![Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211]](https://resources.rndsystems.com/images/products/LEMD3-Antibody-OTI6C10-Western-Blot-NBP2-02211-img0003.jpg "Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211]")
Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211]
Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEMD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEMD3.![Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211]](https://resources.rndsystems.com/images/products/LEMD3-Antibody-OTI6C10-Immunohistochemistry-Paraffin-NBP2-02211-img0004.jpg "Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211]")
Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211]
Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-LEMD3mouse monoclonal antibody.![Flow Cytometry: LEMD3 Antibody (OTI6C10) [NBP2-02211]](https://resources.rndsystems.com/images/products/LEMD3-Antibody-OTI6C10-Flow-Cytometry-NBP2-02211-img0001.jpg "Flow Cytometry: LEMD3 Antibody (OTI6C10) [NBP2-02211]")
Flow Cytometry: LEMD3 Antibody (OTI6C10) [NBP2-02211]
Flow Cytometry: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Analysis of Hela cells, using anti-LEMD3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).![Immunoprecipitation: LEMD3 Antibody (OTI6C10) [NBP2-02211]](https://resources.rndsystems.com/images/products/LEMD3-Antibody-OTI6C10-Immunoprecipitation-NBP2-02211-img0008.jpg "Immunoprecipitation: LEMD3 Antibody (OTI6C10) [NBP2-02211]")
Immunoprecipitation: LEMD3 Antibody (OTI6C10) [NBP2-02211]
Immunoprecipitation: LEMD3 Antibody (OTI6C10) [NBP2-02211] - (Negative control: IP without adding anti-LEMD3 antibody). For each experiment, 500ul of DDK tagged LEMD3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LEMD3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.Applications for LEMD3 Antibody (OTI6C10)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Immunoprecipitation
2-4ug/mg
Western Blot
1:1000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.56 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: LEMD3
Alternate Names
inner nuclear membrane protein Man1, integral inner nuclear membrane protein, LEM domain containing 3, MAN1LEM domain-containing protein 3
Entrez Gene IDs
495 (Human)
Gene Symbol
ATP4A
Additional LEMD3 Products
Product Documents for LEMD3 Antibody (OTI6C10)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LEMD3 Antibody (OTI6C10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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