LEPRE1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-30424

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LEPRE1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: AVECAKTYLLFFPNDEVMNQNLAYYAAMLGEEHTRSIGPRESAKEYRQRSLLEKELLFFAYDVFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVRISQEIGNLMKEIETLVEEKTKESLDVSRLT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LEPRE1 Antibody - BSA Free

Western Blot: LEPRE1 Antibody [NBP2-30424]

Western Blot: LEPRE1 Antibody [NBP2-30424]

Western Blot: LEPRE1 Antibody [NBP2-30424] - Analysis in human cell lines U2OS and MCF-7 using anti-P3H1 antibody. Corresponding P3H1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunohistochemistry-Paraffin: LEPRE1 Antibody [NBP2-30424]

Immunohistochemistry-Paraffin: LEPRE1 Antibody [NBP2-30424]

Immunohistochemistry-Paraffin: LEPRE1 Antibody [NBP2-30424] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: LEPRE1 Antibody [NBP2-30424]

Immunohistochemistry-Paraffin: LEPRE1 Antibody [NBP2-30424]

Immunohistochemistry-Paraffin: LEPRE1 Antibody [NBP2-30424] - Staining of human cervix shows weak to moderate cytoplasmic positivity in squamous epithelial cells.
LEPRE1 Antibody LEPRE1 Antibody

LEPRE1 Antibody [NBP2-30424] - staining of human placenta shows strong cytoplasmic positivity in decidual cells.

LEPRE1 Antibody [NBP2-30424] - staining of human placenta shows strong cytoplasmic positivity in decidual cells.
LEPRE1 Antibody LEPRE1 Antibody

LEPRE1 Antibody [NBP2-30424] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.

LEPRE1 Antibody [NBP2-30424] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
LEPRE1 Antibody LEPRE1 Antibody

LEPRE1 Antibody [NBP2-30424] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

LEPRE1 Antibody [NBP2-30424] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Applications for LEPRE1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LEPRE1

LEPRE1 encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Alternate Names

EC 1.14.11.7, GROS1OI8, Growth suppressor 1, leprecan, Leprecan-1, Leucine- and proline-enriched proteoglycan 1, leucine proline-enriched proteoglycan (leprecan) 1, MGC117314, P3H1LEPRECAN, prolyl 3-hydroxylase 1

Gene Symbol

P3H1

UniProt

Q32P28 (Human)

Additional LEPRE1 Products

Product Documents for LEPRE1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for LEPRE1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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