Canine Leptin/OB ELISA Kit (Colorimetric)

Key Product Details

Product Specifications

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Recovery for Canine Leptin/OB ELISA Kit (Colorimetric)

Kit Contents for Canine Leptin/OB ELISA Kit (Colorimetric)

• Assay Diluent A
• Assay Diluent B
• Detection Reagent A
• Detection Reagent B
• Instruction manual
• Plate sealer for 96 wells
• Pre-coated ready to use 96-well strip plate
• Standard
• Standard Diluent
• Stop Solution
• TMB Substrate
• Wash Buffer (30 x concentrate)

Preparation and Storage

Background: Leptin/OB

Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure. Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.

Additional Leptin/OB Products

• All Products for Leptin/OB
• Leptin/OB ELISA Kits
• Leptin/OB Luminex Assays
• Leptin/OB Lysates
• Leptin/OB Primary Antibodies
• Leptin/OB Proteins and Enzymes
• Leptin/OB Simple Plex

Product Documents for Canine Leptin/OB ELISA Kit (Colorimetric)