Lgr4/GPR48 Antibody (6G8.B3.G5.C3)

Name: Lgr4/GPR48 Antibody (6G8.B3.G5.C3)
Brand: Novus Biologicals
SKU: NBP3-50141
Price: 729 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-50141

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 6G8.B3.G5.C3

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Product Specifications

Immunogen

A synthetic peptide corresponding to an internal region of human Lgr4/GPR48 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

Reactivity Notes

BLAST analysis was used to suggest cross-reactivity with LGR4 from chimpanzee, orangutan and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with LGR4 from other sources has not been determined.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for Lgr4/GPR48 Antibody (6G8.B3.G5.C3)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This antibody has been tested by ELISA and western blot, and is suitable in immunohistochemistry. Expect a band approximately 102 kDa in size corresponding to Lgr4/GPR48 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.

Suggested applications: IHC

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lgr4/GPR48

GPR48 is an Orphan-A GPCR with an unknown ligand. GPR48 expression has been documented in human brain, adrenal, heart, kidney, liver, skeletal muscle, ovary, pancreas, placenta, prostate, spleen, stomach, testis, thyroid, and spinal cord.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 4

Alternate Names

GPR48

Gene Symbol

LGR4

Additional Lgr4/GPR48 Products

Product Documents for Lgr4/GPR48 Antibody (6G8.B3.G5.C3)

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Certificate of Analysis

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Product Specific Notices for Lgr4/GPR48 Antibody (6G8.B3.G5.C3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.