Lgr4/GPR48 Antibody (OTI2D1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72006

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D1

Format

Azide and BSA Free
View all Lgr4/GPR48 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 230-540 of human LGR4 (NP_060960 ) produced in HEK293T Cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Lgr4/GPR48 Antibody (OTI2D1) - Azide and BSA Free

Western Blot: Lgr4/GPR48 Antibody (OTI2D1)Azide and BSA Free [NBP2-72006]

Western Blot: Lgr4/GPR48 Antibody (OTI2D1)Azide and BSA Free [NBP2-72006]

Western Blot: Lgr4/GPR48 Antibody (OTI2D1) - Azide and BSA Free [NBP2-72006] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPR48 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPR48.
Flow Cytometry: Lgr4/GPR48 Antibody (OTI2D1) - Azide and BSA Free [NBP2-72006]

Flow Cytometry: Lgr4/GPR48 Antibody (OTI2D1) - Azide and BSA Free [NBP2-72006]

Flow Cytometry: Lgr4/GPR48 Antibody (OTI2D1) - Azide and BSA Free [NBP2-72006] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-GPR48 antibody, and then analyzed by flow cytometry.

Applications for Lgr4/GPR48 Antibody (OTI2D1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lgr4/GPR48

GPR48 is an Orphan-A GPCR with an unknown ligand. GPR48 expression has been documented in human brain, adrenal, heart, kidney, liver, skeletal muscle, ovary, pancreas, placenta, prostate, spleen, stomach, testis, thyroid, and spinal cord.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 4

Alternate Names

GPR48

Gene Symbol

LGR4

Additional Lgr4/GPR48 Products

Product Documents for Lgr4/GPR48 Antibody (OTI2D1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Lgr4/GPR48 Antibody (OTI2D1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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