lin-54 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-82002
![Western Blot: lin-54 AntibodyBSA Free [NBP2-82002]](https://resources.rndsystems.com/images/products/lin-54-Antibody-Western-Blot-NBP2-82002-img0001.jpg "Western Blot: lin-54 AntibodyBSA Free [NBP2-82002]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
lin-54 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human lin-54. The immunogen is located within the last 50 amino acids of lin-54. Amino Acid Squence: EQADKKGKSKAAAERM
Specificity
lin-54 antibody is human, mouse, and rat reactive. Multiple isoforms of lin-54 are known to exist.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for lin-54 Antibody - BSA Free
Western Blot: lin-54 AntibodyBSA Free [NBP2-82002]
Western Blot: lin-54 Antibody [NBP2-82002] - Analysis of lin-54 in HeLa cell lysate with lin-54 antibody at 1 ug/ml.![Immunocytochemistry/ Immunofluorescence: lin-54 Antibody - BSA Free [NBP2-82002]](https://resources.rndsystems.com/images/products/lin-54-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82002-img0005.jpg "Immunocytochemistry/ Immunofluorescence: lin-54 Antibody - BSA Free [NBP2-82002]")
Immunocytochemistry/ Immunofluorescence: lin-54 Antibody - BSA Free [NBP2-82002]
Immunocytochemistry/Immunofluorescence: lin-54 Antibody [NBP2-82002] - Analysis of lin-54 in HeLa cells with lin-54 antibody at 20 ug/ml. Red: lin-54 antibody [NBP2-82002]. Blue: DAPI staining.
Immunocytochemistry/ Immunofluorescence: lin-54 Antibody - BSA Free [NBP2-82002] -
Immunocytochemistry/ Immunofluorescence: lin-54 Antibody - BSA Free [NBP2-82002] - Immunofluorescence of lin-54 in HeLa cells with lin-54 antibody at 20 u/ml.![Immunocytochemistry/ Immunofluorescence: lin-54 Antibody - BSA Free [NBP2-82002]](https://resources.rndsystems.com/images/products/lin-54-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82002-img0004.jpg "Immunocytochemistry/ Immunofluorescence: lin-54 Antibody - BSA Free [NBP2-82002]")
Immunocytochemistry/ Immunofluorescence: lin-54 Antibody - BSA Free [NBP2-82002]
Immunocytochemistry/Immunofluorescence: lin-54 Antibody [NBP2-82002] - Analysis of lin-54 in HeLa cells with lin-54 antibody at 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: lin-54 Antibody - BSA Free [NBP2-82002] -
Immunocytochemistry/ Immunofluorescence: lin-54 Antibody - BSA Free [NBP2-82002] - Immunocytochemistry of lin-54 in HeLa cells with lin-54 at 2.5 u/mL.Applications for lin-54 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2.5-20 ug/ml
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: lin-54
Alternate Names
CXCDC1, JC8.6, MIP120, Protein LIN-54
Gene Symbol
LIN54
Additional lin-54 Products
Product Documents for lin-54 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for lin-54 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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