LMCD1 Antibody (OTI1H5)

Novus Biologicals | Catalog # NBP2-00934

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H5
View all LMCD1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LMCD1(NP_055398) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LMCD1 Antibody (OTI1H5)

Western Blot: LMCD1 Antibody (OTI1H5) [NBP2-00934]

Western Blot: LMCD1 Antibody (OTI1H5) [NBP2-00934]

Western Blot: LMCD1 Antibody (1H5) [NBP2-00934] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1.
Immunocytochemistry/Immunofluorescence: LMCD1 Antibody (1H5) [NBP2-00934] Staining of COS7 cells transiently transfected by pCMV6-ENTRY LMCD1.
Immunohistochemistry-Paraffin: LMCD1 Antibody (OTI1H5) [NBP2-00934]

Immunohistochemistry-Paraffin: LMCD1 Antibody (OTI1H5) [NBP2-00934]

Immunohistochemistry-Paraffin: LMCD1 Antibody (1H5) [NBP2-00934] - Staining of paraffin-embedded Human lymphoma tissue using anti-LMCD1 mouse monoclonal antibody.
Flow Cytometry: LMCD1 Antibody (OTI1H5) [NBP2-00934]

Flow Cytometry: LMCD1 Antibody (OTI1H5) [NBP2-00934]

Flow Cytometry: LMCD1 Antibody (1H5) [NBP2-00934] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMCD1 antibody, and then analyzed by flow cytometry.

Applications for LMCD1 Antibody (OTI1H5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.8 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LMCD1

The protein encoded by this gene contains a cysteine-rich domain in the N-terminal region and 2 LIM domains in the C-terminal region. It also has several potential phosphorylation and N-myristoylation sites and a single potential N-glycosylation site. The presence of LIM domains implies involvement in protein-protein interactions. Expression of this gene has been detected in most tissues, with highest expression in skeletal muscle. Transcript variants utilizing alternative polyA signals have been observed. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Alternate Names

dyxin, LIM and cysteine-rich domains 1, LIM and cysteine-rich domains protein 1

Entrez Gene IDs

29995 (Human)

Gene Symbol

LMCD1

Additional LMCD1 Products

Product Documents for LMCD1 Antibody (OTI1H5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LMCD1 Antibody (OTI1H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LMCD1 Antibody (OTI1H5)

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Protocols

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