LOK Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13396

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LOK Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: TLENHTQNSSEVSPPSLNADKPLEESPSTPLAPSQSQDSVNEPCSQPSGDRSLQTTSPPVVAPGNENGLAVPVPLRKSRPVSMDARIQVAQEKQVAEQGG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LOK Antibody - BSA Free

Immunohistochemistry-Paraffin: LOK Antibody [NBP2-13396]

Immunohistochemistry-Paraffin: LOK Antibody [NBP2-13396]

Immunohistochemistry-Paraffin: LOK Antibody [NBP2-13396] - Staining of human rectum shows strong membranous positivity in lymphoid cells.
Immunohistochemistry-Paraffin: LOK Antibody [NBP2-13396]

Immunohistochemistry-Paraffin: LOK Antibody [NBP2-13396]

Immunohistochemistry-Paraffin: LOK Antibody [NBP2-13396] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: LOK Antibody [NBP2-13396]

Immunohistochemistry-Paraffin: LOK Antibody [NBP2-13396]

Immunohistochemistry-Paraffin: LOK Antibody [NBP2-13396] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: LOK Antibody [NBP2-13396]

Immunohistochemistry-Paraffin: LOK Antibody [NBP2-13396]

Immunohistochemistry-Paraffin: LOK Antibody [NBP2-13396] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.
LOK Antibody - BSA Free Immunohistochemistry: LOK Antibody - BSA Free [NBP2-13396]

Immunohistochemistry: LOK Antibody - BSA Free [NBP2-13396]

Analysis in human tonsil and skeletal muscle tissues using NBP2-13396 antibody. Corresponding STK10 RNA-seq data are presented for the same tissues.
LOK Antibody - BSA Free Western Blot: LOK Antibody - BSA Free [NBP2-13396]

Western Blot: LOK Antibody - BSA Free [NBP2-13396]

Analysis in human cell lines SK-MEL-30 and HeLa using Anti-STK10 antibody. Corresponding STK10 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Applications for LOK Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LOK

LOK encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and over expression of a kinase dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase 1 pathway.

Alternate Names

EC 2.7.11, EC 2.7.11.1, LOKserine/threonine-protein kinase 10, Lymphocyte-oriented kinase, PRO2729, serine/threonine kinase 10

Gene Symbol

STK10

Additional LOK Products

Product Documents for LOK Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for LOK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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