Loricrin Antibody

Novus Biologicals | Catalog # NBP1-33610

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Rhesus Macaque (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Loricrin Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Loricrin. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (87%).

Localization

Cytoplasm, Nucleus, nucleoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Loricrin Antibody

Immunohistochemistry: Loricrin Antibody [NBP1-33610]

Immunohistochemistry: Loricrin Antibody [NBP1-33610]

Immunohistochemistry: Loricrin Antibody [NBP1-33610] - Prominent differences in the expressions of innate immune and barrier molecules between sebaceous gland poor (SGR) and sebaceous gland rich (SGP) skin regions. Representative images for immunostaining and quantification of epidermal levels of LOR in SGP and SGR skin sections. Images of negative control stainings are shown in the bottom left corner of SGP immunostainings. Size bars=100um. The graphs show the mean +/- SEM of measured protein levels (*p<0.05; **p<0.01; ***p<0.001, as determined by Mann-Whitney U-test). Image collected and cropped by CiteAb from the following publication (frontiersin.org/articles/10.3389/fimmu.2018.00424/full), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Loricrin Antibody [NBP1-33610]

Immunocytochemistry/ Immunofluorescence: Loricrin Antibody [NBP1-33610]

Immunocytochemistry/Immunofluorescence: Loricrin Antibody [NBP1-33610] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.
Western Blot: Loricrin Antibody [NBP1-33610]

Western Blot: Loricrin Antibody [NBP1-33610]

Western Blot: Loricrin Antibody [NBP1-33610] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Loricrin antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Loricrin Antibody [NBP1-33610]

Immunohistochemistry-Paraffin: Loricrin Antibody [NBP1-33610]

Immunohistochemistry-Paraffin: Loricrin Antibody [NBP1-33610] - HBL438 xenograft. Loricrin antibody [N1], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Loricrin Antibody [NBP1-33610]

Immunohistochemistry-Paraffin: Loricrin Antibody [NBP1-33610]

Immunohistochemistry-Paraffin: Loricrin Antibody [NBP1-33610] - Reconstructed human epidermis tissues were embedded in paraffin. After successives baths in xylene and ethanol, antigen was retrieved using warm citrate (for 30 min). Specimens were incubated overnight with the primary antibody (1:100 dilution). Blue staining corresponds to nuclei while loricrin is visible in green. IHC-P image submitted by a verified customer review.
Simple Western: Loricrin Antibody [NBP1-33610]

Simple Western: Loricrin Antibody [NBP1-33610]

Simple Western: Loricrin Antibody [NBP1-33610] - NHEK human normal epidermal keratinocytes. Antibody dilution of 1:50. Protein concentration of 450 ug/mL. Detection is chemiluminescence. Simple Western image submitted by a verified customer review.
Loricrin Antibody

Western Blot: Loricrin Antibody [NBP1-33610] -

Western Blot: Loricrin Antibody [NBP1-33610] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Loricrin antibody [N1], N-term (NBP1-33610) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Loricrin Antibody

Western Blot: Loricrin Antibody [NBP1-33610] -

The effect of YAP expression on skin barrier in AD mice. (A) Trans epidermal water loss (TEWL) and stratum corneum hydration (SCH) of each group. (B) YAP protein expression from immunohistochemistry with the quantification results shown in the graphs (×400), bar length = 50 μm. n = 3. (C) Filaggrin (FLG) protein expression from immunohistochemistry with the quantification results shown in the graphs (×400), bar length = 50 μm. n = 3. (D) FLG, involucrin (IVL), and loricrin (LOR) protein expression from western blot with the quantification results shown in the graphs. (E) YAP, p-YAP, mTOR, and p-mTOR protein expression from western blot with the quantification results shown in the graphs. Ctrl, control mice; AD, atopic dermatitis model mice; BLK, mice injected with empty vector lentivirus; YAP, mice injected with YAP overexpression lentivirus; AD+YAP, atopic dermatitis model mice injected with YAP overexpression lentivirus; sh-NC, mice injected with shRNA-NC lentivirus; sh-YAP, mice injected with YAP shRNA lentivirus; AD+sh-YAP, atopic dermatitis model mice injected with YAP shRNA lentivirus; AD+sh-YAP+RPM, AD+sh-YAP group with topical 0.2% rapamycin ointment. *P < 0.05, ***P < 0.001 Ctrl v.s. AD; #P < 0.05, ##P < 0.01, ###P < 0.001 AD+YAP v.s. AD; +P < 0.05, +++P < 0.001 AD+sh-YAP v.s. AD; ^^P < 0.01, ^^^P < 0.001 AD+sh-YAP+RPM v.s. AD+sh-YAP. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fimmu.2025.1681148/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Loricrin Antibody

Western Blot: Loricrin Antibody [NBP1-33610] -

The effect of beta -endorphin directly through the u-opioid receptor, and the effect of inhibition of Akt/mTOR signaling on UVB-induced disruption of epidermal homeostasis. (a) NHKs were pre-incubated with 100 nM of CTOP for 30 min before 15 mJ/cm2 UVB irradiation and the addition of 100 nM beta -endorphin. (b, c) Representative immunoblots and the quantification of the phosphorylation level of proteins in Akt/mTOR signaling pathway (b) and expression levels of differentiation markers (c) showing the reversal effect of beta -endorphin is suppressed by 100 nM CTOP treatment and the reversal effect of 500 nM rapamycin treatment in NHKs for 30 min before 15 mJ/cm2 UVB irradiation. The original blots are presented in Supplementary Fig. S6. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the designated group. n.s. means not significant. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-023-49886-5), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Loricrin Antibody

Western Blot: Loricrin Antibody [NBP1-33610] -

Treatment with beta -endorphin salvaged UVB irradiation-induced increased proliferation and reduced the expression of epidermal differentiation markers in NHKs. (a) Representative images of 5-ethynyl-2ʹ-deoxyuridine (EdU)-positive cells in keratinocytes after UVB irradiation, followed by beta -endorphin treatment for 24 h. DAPI: 4′,6-diamidino-2-phenylindole. The histogram shows the quantification of EdU as the percentage of cells with positive staining. (b) NHKs were exposed to 15 mJ/cm2 of UVB light, followed by 100 nM beta -endorphin treatment for 48 h. RNA was isolated, and the mRNA expression of loricrin, involucrin, filaggrin, keratin 1, and keratin 10 was analyzed using RT-qPCR. Each mRNA level was normalized to that of the ribosomal gene ribosomal protein L13a (RPL13A). (c) Representative immunoblots showing differentiation markers expression levels. The original blots are presented in Supplementary Fig. S4. The protein expression levels of differentiation markers were determined via western blot analysis, and the quantification of these proteins is shown in the histogram. Data are presented as the mean +/- SEM of six independent experiments. # p < 0.05, ##p<0.01, ### p < 0.001 compared to the non-irradiated group, and * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the irradiated vehicle-treated group. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-023-49886-5), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Loricrin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Simple Western

1:50

Western Blot

1:500-1:3000
Application Notes

See Simple Western Antibody Database for Simple Western validation: Tested in NHEK human normal epidermal keratinocytes, separated by Size, antibody dilution of 1:50

Reviewed Applications

Read 3 reviews rated 4.3 using NBP1-33610 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Loricrin

This gene encodes loricrin, a major protein component of the cornified cell envelope found in terminally differentiated epidermal cells. Mutations in this gene are associated with Vohwinkel's syndrome and progressive symmetric erythrokeratoderma, both inherited skin diseases. [provided by RefSeq]

Alternate Names

loricrin, LRN, MGC111513

Gene Symbol

LORICRIN

Additional Loricrin Products

Product Documents for Loricrin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Loricrin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Loricrin Antibody

Customer Reviews for Loricrin Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Loricrin Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 of 3 reviews Showing All
Filter By:
Clear Filters X
  • Loricrin Antibody
    Name: Agnès Tessier
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Skin tissue
    Species: Human
    Verified Customer | Posted 02/27/2020
    Reconstructed human epidermis tissues were embedded in paraffin. After successives baths in xylene and ethanol, antigen was retrieved using warm citrate (for 30 min). After a step of saturation, spécimens were incubated overnight with the primary antibody (1:100 dilution). Blue staining corresponds to nuclei while loricrin is visible in green.
    Loricrin Antibody NBP1-33610
  • Loricrin Antibody
    Name: Irini Dijkhoff
    Application: Simple Western
    Sample Tested: NHEK human normal epidermal keratinocytes
    Species: Human
    Verified Customer | Posted 05/24/2019
    Antibody dilution of 1:50. Protein concentration of 450 ug/mL. Detection is chemiluminescence.
    Standard JESS run default settings.
    Loricrin Antibody NBP1-33610
  • Loricrin Antibody
    Name: David Krull
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human skin
    Species: Human
    Verified Customer | Posted 12/08/2016
    Loricrin Antibody NBP1-33610

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies