LOX propeptide Antibody - BSA Free

Novus Biologicals | Catalog # NB110-41568

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LOX propeptide Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region of mouse LOX propeptide (residues 78-115). [UniProt# P28301]

Specificity

This antibody specifically recognizes both the pro-enzyme and pro-peptide forms of LOX protein in mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LOX propeptide Antibody - BSA Free

Western Blot: LOX propeptide AntibodyBSA Free [NB110-41568]

Western Blot: LOX propeptide AntibodyBSA Free [NB110-41568]

Western Blot: LOX propeptide Antibody [NB110-41568] - Detection of LOPP in MC3T3-E1 cell lysate.
Immunohistochemistry: LOX propeptide Antibody - BSA Free [NB110-41568]

Immunohistochemistry: LOX propeptide Antibody - BSA Free [NB110-41568]

LOX-propeptide-Antibody-Immunohistochemistry-NB110-41568-img0017.jpg
Western Blot: LOX propeptide AntibodyBSA Free [NB110-41568]

Western Blot: LOX propeptide AntibodyBSA Free [NB110-41568]

Western Blot: LOX propeptide Antibody - BSA Free [NB110-41568] - LOX propeptide Antibody [NB110-41568] - Human VSMC were transduced with a lentiviral vector encoding for full-length LOX (pLOX; black bars), LOX-PP (pLOX-PP; grey bars) or with the corresponding empty vector (pLVX; V; white bars). (a) Immunoblots corresponding to mature LOX (mLOX; left panel) & LOX-PP (right panel). The position of the pro-enzyme (ProLOX, right panel), detected with the antibody against the propeptide. GAPDH was analyzed as a loading control. Displayed blots are not cropped from different gels or different parts of the same gel. Representative immunoblots from 3 independent experiments were shown. Image collected & cropped by Cite Ab from the following publication (https://pubmed.ncbi.nlm.nih.gov/30185869/) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: LOX propeptide Antibody - BSA Free [NB110-41568]

Immunocytochemistry/ Immunofluorescence: LOX propeptide Antibody - BSA Free [NB110-41568]

Immunocytochemistry/Immunofluorescence: LOX propeptide Antibody [NB110-41568] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-LOX propeptide at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: LOX propeptide AntibodyBSA Free [NB110-41568]

Western Blot: LOX propeptide AntibodyBSA Free [NB110-41568]

Western Blot: LOX propeptide Antibody [NB110-41568] - Total protein from human and mouse brain was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-LOX propeptide in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: LOX propeptide Antibody - BSA Free [NB110-41568]

Immunohistochemistry-Paraffin: LOX propeptide Antibody - BSA Free [NB110-41568]

Immunohistochemistry-Paraffin: LOX propeptide Antibody [NB110-41568] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse intestine using 1:200 dilution of rabbit anti- LOX propeptide (LOX-PP) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This LOX-PP antibody generated a strong cytoplasmic staining in the epithelial and other cells with a majority of the signal localizing to luminal end of columner epithelial cells. There appears to be some nuclear positivity also which might be a nuclear variant of LOX-PP.
LOX propeptide Antibody - BSA Free

Immunohistochemistry: LOX propeptide Antibody - BSA Free [NB110-41568] -

Immunohistochemistry: LOX propeptide Antibody - BSA Free [NB110-41568] - Mature LOX & LOX-PP were over-expressed in the vascular wall & in VSMC from TgLOX mice. (a) Representative immunohistochemical analysis showing LOX & LOX-PP staining (brown color) in aorta from both wild-type & TgLOX mice. The indicated areas are shown at high magnification (bars = 20 µm). (b,c) Mature LOX & LOX-PP protein levels were determined by Western-blot in VSMC supernatants from these animals. The position of the pro-enzyme (ProLOX), mature LOX (mLOX) & LOX-PP forms are indicated. GAPDH was analyzed as loading control. Representative immunoblots from 3 independent experiments were shown. WT: wild-type; Tg: TgLOX. Displayed blots are not cropped from different gels or different parts of the same gel & images conform the digital image & integrity policies of the journal. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30185869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
LOX propeptide Antibody - BSA Free

Western Blot: LOX propeptide Antibody - BSA Free [NB110-41568] -

Western Blot: LOX propeptide Antibody - BSA Free [NB110-41568] - Over-expression of LOX-PP did not affect VSMC proliferation. Human VSMC were transduced with a lentiviral vector encoding for full-length LOX (pLOX; black bars), LOX-PP (pLOX-PP; grey bars) or with the corresponding empty vector (pLVX; V; white bars). (a) Immunoblots corresponding to mature LOX (mLOX; left panel) & LOX-PP (right panel) are shown. The position of the pro-enzyme (ProLOX, right panel), detected with the antibody against the propeptide, was also indicated. GAPDH was analyzed as a loading control. Displayed blots are not cropped from different gels or different parts of the same gel & images conform the digital image & integrity policies of the journal. Representative immunoblots from 3 independent experiments were shown. (b) Transduced VSMC were serum-starved for 24 h & then stimulated with 20% FCS. Cell proliferation was evaluated by the [3H]-thymidine incorporation method (left panel) or by cell count (right panel) in these cells. Results represented as mean ± SD. *P < 0.003 vs. pLVX (Kruskal-Wallis; at least n = 6). (c) [3H]-thymidine incorporation into DNA assessed in human VSMC transduced with lentiviral particles for full-length LOX (pLOX; black bars), or the corresponding empty vector (pLVX; white bars) treated or not with BAPN (500 µM). Results are represented as mean ± SD. P < 0.0001: * vs. pLVX; #vs. pLOX transduced cells (Two-way ANOVA; at least n = 9). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30185869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for LOX propeptide Antibody - BSA Free

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 26080361)

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

0.5-1.0 ug/ml

Immunohistochemistry-Paraffin

1:100-1:250

Western Blot

1:500-1:2000
Application Notes
In Western Blot, a band is seen at ~18 kDa. The strong band at 50 kDa and smear of bands that run between 46 kDa and 50 kDa are glycosylated and non-glycosylated forms of pro-lysyl oxidase. Bands between 28-36 kDa are glycosylated propeptide. The variation in MW depends on the extent of glycosylation.

Reviewed Applications

Read 2 reviews rated 3.5 using NB110-41568 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LOX propeptide

Lysyl oxidase (LOX) is a critical enzyme in extracellular matrix biosynthesis as well as in the maintainance of normal bone phenotype. It is synthesized/secreted as a 50-kD proenzyme 'pro-LOX' which is then processed to 30-kD mature enzyme 'LOX' and 18-kD lysyl oxidase propeptide 'LOX-PP' by extracellular procollagen C-proteinases (BMP1, Tll1, and Tll2). LOX-PP has been proposed to implicate in the maintenance of inactive state of LOX within the secretory pathway and to act as intramolecular chaperones which facilitate the accurate protein folding/targeting to correct destinations. LOX-PP involves tumor suppression by inhibiting RAS-dependent cellular transformation and its expression has also been associated with inhibition of anchorage-independent progression as well as migration and growth suppression xenografts in Her-2/neu-driven breast cancer. LOX-PP also inhibits PI3K/AKT and ERK1/2 MAP kinase pathways, as well as the levels of downstream NF-kB/cyclin D1 signals in certain cancers - breast, pancreatic, lung, prostate and oral cancers. LOX-PP has also been shown to inhibit DNA synthesis in rat's VSMCs cultures, osteoblast proliferation and FGF-2 signaling.

Alternate Names

lysyl oxidaseEC 1.4.3.13, MGC105112, protein-lysine 6-oxidase

Entrez Gene IDs

16948 (Mouse)

Gene Symbol

LOX

Additional LOX propeptide Products

Product Documents for LOX propeptide Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LOX propeptide Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LOX propeptide Antibody - BSA Free

Customer Reviews for LOX propeptide Antibody - BSA Free (2)

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  • LOX propeptide Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: cardiac tissue
    Species: Mouse
    Verified Customer | Posted 11/29/2016
    Protein was extracted from infarcted tissue by homogenizing the samples sequentially in phosphate buffered saline (PBS) with 1x protease inhibitor cocktail (16 μL per mg tissue, soluble protein fraction), and in protein extraction reagent type 4 (Sigma; 7 M urea, 2 M thiourea, 40 mM Trizma® base and the detergent 1% C7BzO, 15 μL per mg tissue). Immunoblotting was performed with insoluble fraction.
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: supernatants from human vascular smooth muscle cells
    Species: Human
    Verified Customer | Posted 03/14/2014
    LOX propeptide levels analyzed in supernatants from human vascular smooth muscle cells
    LOX propeptide Antibody - BSA Free NB110-41568

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Protocols

View specific protocols for LOX propeptide Antibody - BSA Free (NB110-41568):


Antigen Unmasking
- Bring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes.

Staining
1. Wash sections in dH2O three times for 5 minutes each.
2. Wash section in wash buffer (1X PBS/0.1% Tween-20 (1X PBST)) for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1X PBST, 5% goat serum) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul primary antibody diluted in 1X PBST, 5% goat serum to each section. Incubate overnight at 4C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated secondary antibody, diluted in 1X PBST, 5% goat serum. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in dH2O.
12. Counterstain sections in hematoxylin.
13. Wash sections in dH2O two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

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FAQs for LOX propeptide Antibody - BSA Free

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  • Q: Does that mean it has been tested on frozen sections and didn't work?

    A: This has been validated for both Paraffin and Frozen. In the applications list we list IHC. This includes both IHC-Frozen and IHC-Paraffin.

  • Q: Just wondered whether the LOX propeptide Antibody [NB110-41568] has been tested on frozen sections?

    A: This antibody has been tested on frozen sections.

  • Q: Does that mean it has been tested on frozen sections and didn't work?

    A: This has been validated for both Paraffin and Frozen. In the applications list we list IHC. This includes both IHC-Frozen and IHC-Paraffin.

  • Q: Just wondered whether the LOX propeptide Antibody [NB110-41568] has been tested on frozen sections?

    A: This antibody has been tested on frozen sections.

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