LRCH3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81083

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LRCH3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HASPLPPSAAPTTDSTDSITGQNSRQREEELELIDQLRKHIEYRLKVSLPCDLGAALTDGVVLCHLANHVRPRSVPSIHVPSP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LRCH3 Antibody - BSA Free

Western Blot: LRCH3 Antibody [NBP1-81083]

Western Blot: LRCH3 Antibody [NBP1-81083]

Western Blot: LRCH3 Antibody [NBP1-81083] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: LRCH3 Antibody [NBP1-81083]

Immunocytochemistry/ Immunofluorescence: LRCH3 Antibody [NBP1-81083]

Immunocytochemistry/Immunofluorescence: LRCH3 Antibody [NBP1-81083] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083]

Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083]

Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Western Blot: LRCH3 Antibody [NBP1-81083]

Western Blot: LRCH3 Antibody [NBP1-81083]

Western Blot: LRCH3 Antibody [NBP1-81083] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083]

Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083]

Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083]

Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083]

Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083]

Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083]

Immunohistochemistry-Paraffin: LRCH3 Antibody [NBP1-81083] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Applications for LRCH3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LRCH3

Alternate Names

FLJ20994, FLJ43245, FLJ44976,2210409B11Rik, leucine-rich repeat and calponin homology domain-containing protein 3, leucine-rich repeats and calponin homology (CH) domain containing 3, MGC4126

Entrez Gene IDs

84859 (Human)

Gene Symbol

LRCH3

Additional LRCH3 Products

Product Documents for LRCH3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for LRCH3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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