LRGUK Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: LRGUK Antibody [NBP2-17163]

Immunocytochemistry/Immunofluorescence: LRGUK Antibody [NBP2-17163] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

Western Blot: LRGUK Antibody [NBP2-17163] -

Western Blot: LRGUK Antibody [NBP2-17163] - LRGUK-1 binds to HOOK2.(A) Growth of yeast carrying wild type LRGUK-1 (LRGUKWT/pDEST32) & HOOK2 (HOOK2/pDEST22) compared to those carrying LRGUKKaos (LRGUKKaos/pDEST32) & HOOK2 (HOOK2/pDEST22), & empty pDEST32 & pDEST22 vectors. Binding was absent (i.e. no growth) when empty vectors were used as a negative control. (B) Confirmation of the interaction between LRGUK-1 & HOOK2 by immunoprecipitation from HEK293 cells expressing EGFP-tagged LRGUK-1. EGFP-tagged LRGUK-1 was immunoprecipitated using GFP antibody-conjugated beads (anti-GFP-Trap-A beads), & immunoblotting was performed using HOOK2 antibody to detect the interaction between the EGFP-tagged LRGUK-1 & endogenous HOOK2. pEGFP-C1 represents a negative control which over-expressed EGFP protein only. Input: whole cell lysates of transfected HEK293 cells. (C) The localization of HOOK2 (red) in WT round spermatids, co-labelled with PNA (green) to mark the acrosome. HOOK2 was present within the developing acrosome/acroplaxome (arrowhead), at a region consistent with the leading edge of the acrosome & acroplaxome (arrows) & at a site consistent with the basal body (asterisk). (D) The localisation of HOOK2 (red) onto the microtubules ( alpha -tubulin, green) of the manchette in WT & LrgukKaos/Kaos elongating spermatids. Scale bar = 5 μm. (E) HOOK2 (red) within caudal epididymal sperm. Acetylated (Ac) tubulin was used as an axoneme marker. The asterisk indicates HOOK2 in the sperm basal body. Scale bar = 5 μm. In all images, nuclei are labelled with DAPI (blue). Please see S3 Fig. for negative control images. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25781171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LRGUK Antibody [NBP2-17163] -

Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FLJ32786 antibody [N2C1], Internal (NBP2-17163) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: LRGUK Antibody [NBP2-17163] -

Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FLJ32786 antibody [N2C1], Internal (NBP2-17163) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.