LRP2 Antibody (CD7D5) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NB110-96417]

Immunocytochemistry/Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-LRP2 Antibody (CD7D5) NB110-96417 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) - Azide and BSA Free [NB110-96417]

Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Analysis of a FFPE tissue section of mouse kidney using 1:200 dilution of LRP2 [CD7D5] antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NB110-96417]

Immunocytochemistry/Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were incubated with anti-LRP2 (CD7D5) at 20 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) - Azide and BSA Free [NB110-96417]

Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Staining on human kidney at 1:100, overnight incubation at 4C. Image from a review by a confirmed customer.

Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NB110-96417] -

Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NB110-96417] - ICM & TE progenitors show loss of responsiveness to Hippo signaling manipulation at the same time as they loose responsiveness to positional changes. (D) Dox-inducible DN Lats2-IRES-mCherry transgenic embryos imaged before Dox treatment (top panel) & the same embryo was imaged following 24 hr of Dox live (middle panel) & fixed/stained for lineage markers (bottom panel). A representative embryo is shown for each stage. Live mCherry is shown as an extended focus image, IF stainings shown as single plane images. mCherry positive ICMs in mosaic transgenic embryos circled with a dotted line. Arrow points to a rare ICM cell in a 64 cell stage-induced embryo with weak Cdx2 expression, which also co-expressed an ICM marker. Scale bar: 25 µm. n indicates number of transgenic embryos analyzed. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/22906), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NB110-96417] -

Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NB110-96417] - Impairment of autophagy in Atg7-deficient renal proximal tubular cells. (A) Decrease of Atg7 & increase of LC3-I in 2-month-old Atg7flox/flox;KAP-Cre+ mouse kidney. Atg7 & LC3 Western blot (LC3-I/unconjugated LC3 & LC3-II/lipidated LC3) using whole kidney lysate of Atg7flox/flox;KAP-Cre+ mice. As a control, Atg7flox/flox mouse kidney was employed. The intensity of each band of Atg7 & beta -actin was estimated by densitometry. The ratio of Atg7 to beta -actin was calculated. The amount of Atg7-positive signals in the Atg7flox/flox;KAP-Cre+ mice kidney was about 63% lower than that in the Atg7flox/flox mice kidney (n = 3). Note that LC3-I was increased in the Atg7flox/flox;KAP-Cre+ mouse kidney. (B) The massive accumulation of p62 in kidneys of 2-month old Atg7flox/flox;KAP-Cre+ mouse. The cortico-medullary region of each kidney in 2 month-old Atg7flox/flox;KAP-Cre+ mouse & Atg7flox/flox mouse was recognized with anti-p62 antibody (red). As a marker of renal proximal tubular cells, megalin (green) was employed. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue). (C) Quantification of p62 positive area of 2-month-old Atg7flox/flox;KAP-Cre+ (n = 5) & Atg7flox/flox kidney (n = 4, *** p < 0.01). Data in graphs are expressed as the mean ± SEM. Statistical analyses were performed using a student’s t-test. (D) Age-dependent accumulation of p62 (red) in the Atg7flox/flox;KAP-Cre+ mouse kidney in 1-, 2-, 6-, & 9-month-old Atg7flox/flox;KAP-Cre+ (lower) & Atg7flox/flox (upper) mouse kidney. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31881660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.