LRRFIP1 Antibody

Novus Biologicals | Catalog # NBP1-71835

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all LRRFIP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 500 and 550 of human Leucine Rich Repeat (in FLII) Interacting Protein 1 using the numbering given in entry NP_001131024.1 (GeneID 9208).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LRRFIP1 Antibody

Western Blot: LRRFIP1 Antibody [NBP1-71835]

Western Blot: LRRFIP1 Antibody [NBP1-71835]

Western Blot: LRRFIP1 Antibody [NBP1-71835] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (50 ug) cells. Affinity purified rabbit anti-LRRFIP1 antibody used for WB at 0.04 ug/mL (A) and 0.4 ug/mL (B) and used for IP at 6 ug/mg lysate. LRRFIP1 was also immunoprecipitated by rabbit anti-LRRFIP1 antibodies NBP1-71834 and NBP1-71836, which recognize other epitopes.
Immunocytochemistry/ Immunofluorescence: LRRFIP1 Antibody [NBP1-71835]

Immunocytochemistry/ Immunofluorescence: LRRFIP1 Antibody [NBP1-71835]

Immunocytochemistry/Immunofluorescence: LRRFIP1 Antibody [NBP1-71835] - LRRFIP1 in human fibroblasts (Green). 1:250 dilution in 1X PBS/1% BSA/0.3% Triton X-100. 16 hours, 4C. Image submitted by a verified customer review.
Immunoprecipitation: LRRFIP1 Antibody [NBP1-71835]

Immunoprecipitation: LRRFIP1 Antibody [NBP1-71835]

Immunoprecipitation: LRRFIP1 Antibody [NBP1-71835] - LRRFIP1 IP in B-cell whole cell lysate. 2 ug antibody : 500 ug whole cell lysate. Same antibody used at 1:2000 for Western blot. Image submitted by a verified customer review.

Applications for LRRFIP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

From verified customer review.

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000

Reviewed Applications

Read 3 reviews rated 4.3 using NBP1-71835 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LRRFIP1

Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA

Alternate Names

FLAP-1, FLIIAP1, GC-binding factor 2, GCF2, GCF-2, HUFI-1, leucine rich repeat (in FLII) interacting protein 1, leucine-rich repeat flightless-interacting protein 1, LRR FLII-interacting protein 1, MGC10947, MGC119738, MGC119739, NEDD8-conjugating enzyme, TRIPTAR RNA-interacting protein

Gene Symbol

LRRFIP1

UniProt

NP_001131024.1 (Human)

Additional LRRFIP1 Products

Product Documents for LRRFIP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LRRFIP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for LRRFIP1 Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • LRRFIP1 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: B-cell whole cell lysate
    Species: Human
    Verified Customer | Posted 11/20/2018
    LRRFIP1 IP in B-cells
    2ug antibody : 500ug whole cell lysate. Same antibody used 1:2000 for western blot.
    LRRFIP1 Antibody NBP1-71835
  • LRRFIP1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 01/11/2018
    LRRFIP1 in human fibroblasts
    1:250 dilution in 1X PBS/1% BSA/0.3% Triton X-100. 16 hours, 4C.
    LRRFIP1 Antibody NBP1-71835
  • LRRFIP1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: human lymphocytes, whole cell lysate
    Species: Human
    Verified Customer | Posted 12/12/2017
    25ug. 1:2000 dilution in milk, 16H 4C.
    25ug. 1:2000 dilution in milk, 16H 4C.
    LRRFIP1 Antibody NBP1-71835

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