LTB4 Parameter Assay Kit

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Multi-species Leukotriene B4/LTB4 ELISA Standard Curve
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Product Details
Citations (36)
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LTB4 Parameter Assay Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
10.9 pg/mL
Assay Range
10.3 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Measures LTB4 levels in various samples
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
No significant interference observed with available related molecules.

Product Summary

The Parameter LTB4 Immunoassay is a 4.5 hour forward sequential competitive enzyme immunoassay designed to measure LTB4 in cell culture supernates, serum, and plasma.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 170 481 847 164 516 879
Standard Deviation 10 19 34 14.1 36.6 73.6
CV% 5.8 4 4 8.6 7.1 8.4


The recovery of LTB4 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 86-114
EDTA Plasma (n=4) 91 82-112
Heparin Plasma (n=4) 98 82-115
Serum (n=4) 98 82-118


To assess the linearity of the assay, samples spiked with high concentrations of LTB4 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Multi-species Leukotriene B4/LTB4 ELISA Linearity

Data Examples

Multi-species Leukotriene B4/LTB4 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Leukotriene B4/LTB4

Leukotriene B4 (LTB4) is a major product of arachidonic acid metabolism and is formed via the 5-lipoxygenase pathway. LTB4 stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation.

Alternate Names:
Leukotriene B4; LTB4
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Citations for LTB4 Parameter Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Chemotaxis and swarming in differentiated HL-60 neutrophil-like cells
    Authors: KA Babatunde, X Wang, A Hopke, N Lannes, PY Mantel, D Irimia
    Scientific Reports, 2021;11(1):778.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Danggui-Shaoyao-San improves cognitive impairment through inhibiting O-GlcNAc-modification of estrogen alpha receptor in female db/db mice
    Authors: JJ Shi, HF Liu, T Hu, X Gao, YB Zhang, WR Li, Q Wang, SJ Zhang, D Tang, YB Chen
    Journal of ethnopharmacology, 2021;281(0):114562.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. Increased intestinal permeability with elevated peripheral blood endotoxin and inflammatory indices for e-waste lead exposure in children
    Authors: X Luo, X Huo, Y Zhang, Z Cheng, S Chen, X Xu
    Chemosphere, 2021;279(0):130862.
    Species: Human
    Sample Types: Serum
  4. TICAM2-related pathway mediates neutrophil exhaustion
    Authors: R Lin, Y Zhang, K Pradhan, L Li
    Sci Rep, 2020;10(1):14397.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. S1PR4 ablation reduces tumor growth and improves chemotherapy via CD8+ T cell expansion
    Authors: C Olesch, E Sirait-Fis, M Berkefeld, AF Fink, RM Susen, B Ritter, BE Michels, D Steinhilbe, FR Greten, R Savai, K Takeda, B Brüne, A Weigert
    J. Clin. Invest., 2020;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Minocycline microspheres did not significantly improve outcomes after collagenase injection of tendon
    Authors: AD Allen, AM Bassil, DJ Berkoff, M Al Maliki, RW Draeger, PS Weinhold
    J Orthop, 2019;16(6):580-584.
    Species: Rat
    Sample Types: Tissue Culture Supernates
  7. Novel reprogramming of neutrophils modulates inflammation resolution during atherosclerosis
    Authors: S Geng, Y Zhang, C Lee, L Li
    Sci Adv, 2019;5(2):eaav2309.
    Species: Mouse
    Sample Types: Plasma
  8. Serum leukotriene B4 levels, tonsillar hypertrophy and sleep-disordered breathing in childhood
    Authors: E? Alexopoulo, G Haritos, C Befani, G Malakasiot, VA Lachanas, P Liakos, K Gourgoulia, AG Kaditis
    Int. J. Pediatr. Otorhinolaryngol., 2018;113(0):218-222.
    Species: Human
    Sample Types: Serum
  9. Complement factor 5 blockade reduces porcine myocardial infarction size and improves immediate cardiac function
    Authors: SE Pischke, A Gustavsen, HL Orrem, KH Egge, F Courivaud, H Fontenelle, A Despont, AK Bongoni, R Rieben, TI Tønnessen, MA Nunn, H Scott, H Skulstad, A Barratt-Du, TE Mollnes
    Basic Res. Cardiol, 2017;112(3):20.
    Species: Porcine
    Sample Types: Plasma
  10. Let-7 microRNA-dependent control of leukotriene signaling regulates the transition of hematopoietic niche in mice
    Authors: X Jiang, JS Hawkins, J Lee, CO Lizama, FL Bos, JP Zape, P Ghatpande, Y Peng, J Louie, G Lagna, AC Zovein, A Hata
    Nat Commun, 2017;8(1):128.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Early Production of the Neutrophil-Derived Lipid Mediators LTB4 and LXA4 Is Modulated by Intracellular Infection with Leishmania major
    Authors: M Plagge, T Laskay
    Biomed Res Int, 2017;2017(0):2014583.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Disrupting ceramide-CD300f interaction prevents septic peritonitis by stimulating neutrophil recruitment
    Authors: K Izawa, A Maehara, M Isobe, Y Yasuda, M Urai, Y Hoshino, K Ueno, T Matsukawa, M Takahashi, A Kaitani, E Shiba, A Takamori, S Uchida, K Uchida, K Maeda, N Nakano, Y Yamanishi, T Oki, D Voehringer, A Roers, S Nakae, J Ishikawa, Y Kinjo, T Shimizu, H Ogawa, K Okumura, T Kitamura, J Kitaura
    Sci Rep, 2017;7(1):4298.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Suppressive Effect of the n-Hexane Extract of Litsea japonica Fruit Flesh on Monosodium-Iodoacetate-Induced Osteoarthritis in Rats
    Authors: SH Kim, HJ Choi, WK Yang, JE Lee, JH Cho, IJ Park, S Park, BK Park, M Jin
    Evid Based Complement Alternat Med, 2017;2017(0):1791403.
    Species: Rat
    Sample Types: Serum
  14. The development of novel LTA4H modulators to selectively target LTB4 generation
    Authors: CM Low, S Akthar, DF Patel, S Löser, CT Wong, PL Jackson, JE Blalock, SA Hare, CM Lloyd, RJ Snelgrove
    Sci Rep, 2017;7(0):44449.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Inflammatory Mediators in Tracheal Aspirates of Preterm Infants Participating in a Randomized Trial of Inhaled Nitric Oxide
    Authors: M Laube, E Amann, U Uhlig, Y Yang, HW Fuchs, M Zemlin, JC Mercier, RF Maier, HD Hummler, S Uhlig, UH Thome
    PLoS ONE, 2017;12(1):e0169352.
    Species: Human
    Sample Types: Tissue Secretion
  16. The BLT1 Inhibitory Function of alpha-1 Antitrypsin Augmentation Therapy Disrupts Leukotriene B4 Neutrophil Signaling.
    Authors: O'Dwyer C, O'Brien M, Wormald M, White M, Banville N, Hurley K, McCarthy C, McElvaney N, Reeves E
    J Immunol, 2015;195(8):3628-41.
    Species: Human
    Sample Types: Plasma
  17. Zileuton, 5-lipoxygenase inhibitor, acts as a chemopreventive agent in intestinal polyposis, by modulating polyp and systemic inflammation.
    Authors: Gounaris E, Heiferman M, Heiferman J, Shrivastav M, Vitello D, Blatner N, Knab L, Phillips J, Cheon E, Grippo P, Khazaie K, Munshi H, Bentrem D
    PLoS ONE, 2015;10(3):e0121402.
    Species: Human
    Sample Types: Tissue Homogenates
  18. Lysosomal phospholipase A2: a novel player in host immunity to Mycobacterium tuberculosis.
    Authors: Schneider B, Behrends J, Hagens K, Harmel N, Shayman J, Schaible U
    Eur J Immunol, 2014;44(8):2394-404.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. Endogenous omega-3 polyunsaturated fatty acid production confers resistance to obesity, dyslipidemia, and diabetes in mice.
    Authors: Li J, Li F, Wei D, Jia W, Kang J, Stefanovic-Racic M, Dai Y, Zhao A
    Mol Endocrinol, 2014;28(8):1316-28.
    Species: Mouse
    Sample Types: Serum
  20. Role of leukotriene A4 hydrolase aminopeptidase in the pathogenesis of emphysema.
    Authors: Paige M, Wang K, Burdick M, Park S, Cha J, Jeffery E, Sherman N, Shim Y
    J Immunol, 2014;192(11):5059-68.
    Species: Mouse
    Sample Types: BALF
  21. Leukotriene B4 levels in human atherosclerotic plaques and abdominal aortic aneurysms.
    Authors: van den Borne P, van der Laan S, Bovens S, Koole D, Kowala M, Michael L, Schoneveld A, van de Weg S, Velema E, de Vries J, de Borst G, Moll F, de Kleijn D, Quax P, Hoefer I, Pasterkamp G
    PLoS ONE, 2014;9(1):e86522.
    Species: Human
    Sample Types: Tissue Homogenates
  22. Phosphoinositide 3-kinase inhibition restores neutrophil accuracy in the elderly: toward targeted treatments for immunosenescence.
    Authors: Sapey E, Greenwood H, Walton G, Mann E, Love A, Aaronson N, Insall R, Stockley R, Lord J
    Blood, 2014;123(2):239-48.
    Species: Human
    Sample Types: Sputum
  23. The Src family kinases Hck, Fgr, and Lyn are critical for the generation of the in vivo inflammatory environment without a direct role in leukocyte recruitment.
    Authors: Kovacs M, Nemeth T, Jakus Z, Sitaru C, Simon E, Futosi K, Botz B, Helyes Z, Lowell C, Mocsai A
    J Exp Med, 2014;211(10):1993-2011.
    Species: Mouse
    Sample Types: Synovial Fluid
  24. Protective role of acetylsalicylic acid in experimental Trypanosoma cruzi infection: evidence of a 15-epi-lipoxin A(4)-mediated effect.
    Authors: Molina-Berrios A, Campos-Estrada C, Henriquez N, Faundez M, Torres G, Castillo C, Escanilla S, Kemmerling U, Morello A, Lopez-Munoz R, Maya J
    PLoS Negl Trop Dis, 2013;7(4):e2173.
    Species: Mouse
    Sample Types: Plasma
  25. Usage of sphingosine kinase isoforms in mast cells is species and/or cell type determined.
    Authors: Dillahunt S, Sargent J, Suzuki R, Proia R, Gilfillan A, Rivera J, Olivera A
    J Immunol, 2013;190(5):2058-67.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. Circulating leukotriene B4 identifies respiratory complications after trauma.
    Authors: Auner B, Geiger EV, Henrich D, Lehnert M, Marzi I, Relja B
    Mediators Inflamm., 2012;2012(0):536156.
    Species: Human
    Sample Types: Plasma
  27. Ornithodoros moubata complement inhibitor is an equally effective C5 inhibitor in pigs and humans.
    Authors: Barratt-Due A, Thorgersen EB, Lindstad JK, Pharo A, Lissina O, Lambris JD, Nunn MA, Mollnes TE
    J. Immunol., 2011;187(9):4913-9.
    Species: Human
    Sample Types: Plasma
  28. Abscisic acid regulates inflammation via ligand-binding domain-independent activation of peroxisome proliferator-activated receptor gamma.
    Authors: Bassaganya-Riera J, Guri AJ, Lu P, Climent M, Carbo A, Sobral BW, Horne WT, Lewis SN, Bevan DR, Hontecillas R
    J. Biol. Chem., 2011;286(4):2504-16.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  29. Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
    Authors: Chou RC, Kim ND, Sadik CD
    Immunity, 2010;33(2):266-78.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  30. MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
    Authors: Narasaraju T, Ng HH, Phoon MC
    Am. J. Respir. Cell Mol. Biol., 2010;42(6):732-43.
    Species: Mouse
    Sample Types: Serum
  31. Participation of 5-lipoxygenase-derived LTB(4) in 4-hydroxynonenal-enhanced MMP-2 production in vascular smooth muscle cells.
    Authors: Seo KW, Lee SJ, Kim CE
    Atherosclerosis, 2010;208(1):56-61.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  32. Leukotriene B4 enhances the activity of nuclear factor-kappaB pathway through BLT1 and BLT2 receptors in atherosclerosis.
    Authors: Sanchez-Galan E, Gomez-Hernandez A, Vidal C, Martin-Ventura JL, Blanco-Colio LM, Munoz-Garcia B, Ortega L, Egido J, Tuñón J
    Cardiovasc. Res., 2009;81(1):216-25.
    Species: Human
    Sample Types: Plasma
  33. CPAP decreases plasma levels of soluble tumour necrosis factor-alpha receptor 1 in obstructive sleep apnoea.
    Authors: Arias MA, Garcia-Rio F, Alonso-Fernandez A, Hernanz A, Hidalgo R, Martinez-Mateo V, Bartolome S, Rodriguez-Padial L
    Eur. Respir. J., 2008;32(4):1009-15.
    Species: Human
    Sample Types: Plasma
  34. Effects of atorvastatin added to inhaled corticosteroids on lung function and sputum cell counts in atopic asthma.
    Authors: Hothersall EJ, Chaudhuri R, McSharry C, Donnelly I, Lafferty J, McMahon AD, Weir CJ, Meiklejohn J, Sattar N, McInnes I, Wood S, Thomson NC
    Thorax, 2008;63(12):1070-5.
    Species: Human
    Sample Types: Sputum
  35. Effect of intensive atorvastatin therapy on prostaglandin E2 levels and metalloproteinase-9 activity in the plasma of patients with non-ST-elevation acute coronary syndrome.
    Authors: Gomez-Hernandez A, Sanchez-Galan E, Ortego M, Martin-Ventura JL, Blanco-Colio LM, Tarin-Vicente N, Jimenez-Nacher JJ, Lopez-Bescos L, Egido J, Tuñón J
    Am. J. Cardiol., 2008;102(1):12-8.
    Species: Human
    Sample Types: Plasma
  36. Improved pulmonary function by acid sphingomyelinase inhibition in a newborn piglet lavage model.
    Authors: von Bismarck P, Wistadt CF, Klemm K, Winoto-Morbach S, Uhlig U, Schutze S, Adam D, Lachmann B, Uhlig S, Krause MF
    Am. J. Respir. Crit. Care Med., 2008;177(11):1233-41.
    Species: Porcine
    Sample Types: BALF


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