Leukotriene B4 (LTB4) is a major product of arachidonic acid metabolism and is formed via the 5-lipoxygenase pathway. LTB4 stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation.
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
10.9 pg/mL
Assay Range
10.3-2500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for LTB4 Parameter Assay Kit
The Parameter LTB4 Immunoassay is a 4.5 hour forward sequential competitive enzyme immunoassay designed to measure LTB4 in cell culture supernates, serum, and plasma.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Species
Multi-Species
Specificity
Measures LTB4 levels in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 170 | 481 | 847 | 164 | 516 | 879 |
| Standard Deviation | 10 | 19 | 34 | 14.1 | 36.6 | 73.6 |
| CV% | 5.8 | 4.0 | 4.0 | 8.6 | 7.1 | 8.4 |
Recovery for LTB4 Parameter Assay Kit
The recovery of LTB4 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 86-114 |
| EDTA Plasma (n=4) | 91 | 82-112 |
| Heparin Plasma (n=4) | 98 | 82-115 |
| Serum (n=4) | 98 | 82-118 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of LTB4 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for LTB4 Parameter Assay Kit
Multi-species Leukotriene B4/LTB4 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Leukotriene B4/LTB4
Alternate Names
LTB4
Additional Leukotriene B4/LTB4 Products
Product Documents for LTB4 Parameter Assay Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for LTB4 Parameter Assay Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for LTB4 Parameter Assay Kit
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