LTB4 Parameter Assay Kit

(16 citations)   
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    10.9 pg/mL
  • Assay Range
    10.3 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Measures LTB4 levels in various samples
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Parameter LTB4 Immunoassay is a 4.5 hour forward sequential competitive enzyme immunoassay designed to measure LTB4 in cell culture supernates, serum, and plasma.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation10193414.136.673.6


The recovery of LTB4 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 86-114
EDTA Plasma (n=4) 91 82-112
Heparin Plasma (n=4) 98 82-115
Serum (n=4) 98 82-118
To assess the linearity of the assay, samples spiked with high concentrations of LTB4 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
LTB4 Parameter Assay Kit
Preparation and Storage
  • Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Leukotriene B4/LTB4
Leukotriene B4 (LTB4) is a major product of arachidonic acid metabolism and is formed via the 5-lipoxygenase pathway. LTB4 stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation.
    • Alternate Names
    Related Research Areas

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 16
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    Sample Type
    1. The BLT1 Inhibitory Function of alpha-1 Antitrypsin Augmentation Therapy Disrupts Leukotriene B4 Neutrophil Signaling.
      Authors: O'Dwyer C, O'Brien M, Wormald M, White M, Banville N, Hurley K, McCarthy C, McElvaney N, Reeves E
      J Immunol, 2015;195(8):3628-41.
      Species: Human
      Sample Type: Plasma
    2. Zileuton, 5-lipoxygenase inhibitor, acts as a chemopreventive agent in intestinal polyposis, by modulating polyp and systemic inflammation.
      Authors: Gounaris E, Heiferman M, Heiferman J, Shrivastav M, Vitello D, Blatner N, Knab L, Phillips J, Cheon E, Grippo P, Khazaie K, Munshi H, Bentrem D
      PLoS ONE, 2015;10(3):e0121402.
      Species: Human
      Sample Type: Homogenized Tissue
    3. Phosphoinositide 3-kinase inhibition restores neutrophil accuracy in the elderly: toward targeted treatments for immunosenescence.
      Authors: Sapey E, Greenwood H, Walton G, Mann E, Love A, Aaronson N, Insall R, Stockley R, Lord J
      Blood, 2014;123(2):239-48.
      Species: Human
      Sample Type: Sputum
    4. Endogenous omega-3 polyunsaturated fatty acid production confers resistance to obesity, dyslipidemia, and diabetes in mice.
      Authors: Li J, Li F, Wei D, Jia W, Kang J, Stefanovic-Racic M, Dai Y, Zhao A
      Mol Endocrinol, 2014;28(8):1316-28.
      Species: Mouse
      Sample Type: Serum
    5. Usage of sphingosine kinase isoforms in mast cells is species and/or cell type determined.
      Authors: Dillahunt S, Sargent J, Suzuki R, Proia R, Gilfillan A, Rivera J, Olivera A
      J Immunol, 2013;190(5):2058-67.
      Species: Mouse
      Sample Type: cell culture supernate
    6. Circulating leukotriene B4 identifies respiratory complications after trauma.
      Authors: Auner B, Geiger EV, Henrich D, Lehnert M, Marzi I, Relja B
      Mediators Inflamm., 2012;2012(0):536156.
      Species: Human
      Sample Type: Plasma
    7. Ornithodoros moubata complement inhibitor is an equally effective C5 inhibitor in pigs and humans.
      Authors: Barratt-Due A, Thorgersen EB, Lindstad JK, Pharo A, Lissina O, Lambris JD, Nunn MA, Mollnes TE
      J. Immunol., 2011;187(9):4913-9.
      Species: Human
      Sample Type: Plasma
    8. Abscisic acid regulates inflammation via ligand-binding domain-independent activation of peroxisome proliferator-activated receptor gamma.
      Authors: Bassaganya-Riera J, Guri AJ, Lu P, Climent M, Carbo A, Sobral BW, Horne WT, Lewis SN, Bevan DR, Hontecillas R
      J. Biol. Chem., 2011;286(4):2504-16.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    9. Participation of 5-lipoxygenase-derived LTB(4) in 4-hydroxynonenal-enhanced MMP-2 production in vascular smooth muscle cells.
      Authors: Seo KW, Lee SJ, Kim CE
      Atherosclerosis, 2010;208(1):56-61.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    10. Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
      Authors: Chou RC, Kim ND, Sadik CD
      Immunity, 2010;33(2):266-78.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    11. MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
      Authors: Narasaraju T, Ng HH, Phoon MC
      Am. J. Respir. Cell Mol. Biol., 2010;42(6):732-43.
      Species: Mouse
      Sample Type: Serum
    12. Leukotriene B4 enhances the activity of nuclear factor-kappaB pathway through BLT1 and BLT2 receptors in atherosclerosis.
      Authors: Sanchez-Galan E, Gomez-Hernandez A, Vidal C, Martin-Ventura JL, Blanco-Colio LM, Munoz-Garcia B, Ortega L, Egido J, Tuñón J
      Cardiovasc. Res., 2009;81(1):216-25.
      Species: Human
      Sample Type: Plasma
    13. CPAP decreases plasma levels of soluble tumour necrosis factor-alpha receptor 1 in obstructive sleep apnoea.
      Authors: Arias MA, Garcia-Rio F, Alonso-Fernandez A, Hernanz A, Hidalgo R, Martinez-Mateo V, Bartolome S, Rodriguez-Padial L
      Eur. Respir. J., 2008;32(4):1009-15.
      Species: Human
      Sample Type: Plasma
    14. Effects of atorvastatin added to inhaled corticosteroids on lung function and sputum cell counts in atopic asthma.
      Authors: Hothersall EJ, Chaudhuri R, McSharry C, Donnelly I, Lafferty J, McMahon AD, Weir CJ, Meiklejohn J, Sattar N, McInnes I, Wood S, Thomson NC
      Thorax, 2008;63(12):1070-5.
      Species: Human
      Sample Type: Sputum
    15. Effect of intensive atorvastatin therapy on prostaglandin E2 levels and metalloproteinase-9 activity in the plasma of patients with non-ST-elevation acute coronary syndrome.
      Authors: Gomez-Hernandez A, Sanchez-Galan E, Ortego M, Martin-Ventura JL, Blanco-Colio LM, Tarin-Vicente N, Jimenez-Nacher JJ, Lopez-Bescos L, Egido J, Tuñón J
      Am. J. Cardiol., 2008;102(1):12-8.
      Species: Human
      Sample Type: Plasma
    16. Improved pulmonary function by acid sphingomyelinase inhibition in a newborn piglet lavage model.
      Authors: von Bismarck P, Wistadt CF, Klemm K, Winoto-Morbach S, Uhlig U, Schutze S, Adam D, Lachmann B, Uhlig S, Krause MF
      Am. J. Respir. Crit. Care Med., 2008;177(11):1233-41.
      Species: Porcine
      Sample Type: BALF
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