LUC7L Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-56401

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PPDWRLESSNGKMASRRSEEKEAGEI

Reactivity Notes

Mouse 88%, Rat 88%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LUC7L Antibody - BSA Free

Western Blot: LUC7L Antibody [NBP2-56401]

Western Blot: LUC7L Antibody [NBP2-56401]

Western Blot: LUC7L Antibody [NBP2-56401] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: LUC7L Antibody [NBP2-56401]

Immunocytochemistry/ Immunofluorescence: LUC7L Antibody [NBP2-56401]

Immunocytochemistry/Immunofluorescence: LUC7L Antibody [NBP2-56401] - Staining of human cell line MCF7 shows localization to nuclear speckles & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LUC7L Antibody [NBP2-56401]

Immunohistochemistry-Paraffin: LUC7L Antibody [NBP2-56401]

Immunohistochemistry-Paraffin: LUC7L Antibody [NBP2-56401] - Staining of human fallopian tube shows nuclear positivity in glandular cells.
LUC7L Antibody - BSA Free

Western Blot: LUC7L Antibody - BSA Free [NBP2-56401] -

JMJD6 forms an interaction network that consists of proteins involved in splicing and protein synthesis.(A) FLAG-tagged JMJD6 transduced into SK-N-AS cells for immunoprecipitation with anti-FLAG followed by protein identification by mass spectrometry. The interacting protein partners of JMJD6 are analyzed by STRING protein network. (B) Immunoprecipitation followed by western blot to validate the JMJD6-interacting partners in SK-N-AS cells. IP = immunoprecipitation, FT = flowthrough. n=single experiment. (C) Click-iT AHA labeling showing the newly synthesized proteins after overexpression of JMJD6 in SK-N-AS cells. n=single experiment. (D, E) Western blot showing the expression of GAC and KGA isoforms in SKNAS (D), BE2C (E), after U2AF2 and CPSF6 knockdown for 72 hr. n=single experiment.Figure 6—source data 1.Mass spectrometric analysis JMJD6 interactomes in BE2C and SKNAS cells.Mass spectrometric analysis JMJD6 interactomes in BE2C and SKNAS cells.The JMJD6 interactome in BE2C cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38488852), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for LUC7L Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LUC7L

The LUC7L gene may represent a mammalian heterochromatic gene, encoding a putative RNA-binding protein similar to the yeast Luc7p subunit of the U1 snRNP splicing complex that is normally required for 5-prime splice site selection (Tufarelli et al., 2001 [PubMed 11170747]).[supplied by OMIM]

Alternate Names

FLJ10231, hLuc7B1, Luc7, LUC7 (S. cerevisiae)-like, LUC7B1, LUC7L1, LUC7-LIKE, LUC7-like (S. cerevisiae), putative RNA-binding protein Luc7-like 1, Putative SR protein LUC7B1, sarcoplasmic reticulum protein LUC7B1, SR+89

Gene Symbol

LUC7L

Additional LUC7L Products

Product Documents for LUC7L Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for LUC7L Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LUC7L Antibody - BSA Free

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Protocols

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