Luciferase Antibody (Luci 21 1-107) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80837

Novus Biologicals
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Key Product Details

Species Reactivity

Firefly

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # Luci 21 1-107

Format

Azide and BSA Free
View all Luciferase Primary Antibodies »

Product Specifications

Immunogen

This Luciferase Antibody (Luci 21 1-107) was developed against luciferase protein from Photinus pyralis (North American firefly). [UniProt# P08659].

Reactivity Notes

Photinus pyralis (North American firefly).

Localization

Peroxisome

Specificity

This Luciferase Antibody (Luci 21 1-107) is specific for Luciferase, recognizing a peptide consisting of the first 258 amino acids. Further epitope mapping has not been done at this time.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Luciferase Antibody (Luci 21 1-107) - Azide and BSA Free

Western Blot: Luciferase Antibody (Luci 21 1-107)Azide and BSA Free [NBP2-80837]

Western Blot: Luciferase Antibody (Luci 21 1-107)Azide and BSA Free [NBP2-80837]

Western Blot: Luciferase Antibody (Luci 21 1-107) - Azide and BSA Free [NBP2-80837] - WB analysis of NB600-307 on Luciferase Protein (NB810-74573) Image from the standard format of this antibody.
Immunohistochemistry: Luciferase Antibody (Luci 21 1-107) - Azide and BSA Free [NBP2-80837]

Immunohistochemistry: Luciferase Antibody (Luci 21 1-107) - Azide and BSA Free [NBP2-80837]

Immunohistochemistry: Luciferase Antibody (Luci 21 1-107) - Azide and BSA Free [NBP2-80837] - Detection of Luciferase expression in CD34+ cells by Immunohistochemistry. Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with monoclonal anti-Luciferase antibody. Luciferase-positive cells have green cytoplasm;

Applications for Luciferase Antibody (Luci 21 1-107) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:200. Use reported by customer review

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Frozen

reported in scientific literature (PMID 31069316)

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:1000
Application Notes
Western blot has been tested with Drosophila embryos. Purified Luciferase protein and Luciferase expressed in Drosophila adult co-migrate on Western blots with a band seen at ~61 kDa, representing Luciferase.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Luciferase

Luciferase is a generic term for a group of oxidative enzymes used in bioluminescence. Firefly (Photinus pyralis) and bacterial luciferase enzymes are commonly used in assay systems such as cell viability assays, reporter gene assays, and for in vivo imaging. Bacterial luciferases are flavoenzymes composed of two subunits each encoded by the luxA and luxB genes, while the firefly luciferase is a single polypeptide specified by the luc gene (1). Firefly luciferase (theoretical molecular weight: 61 kDa) oxidizes the substrate luciferin to oxyluciferin in a bioluminescent reaction requiring Mg2+ and ATP (2,3). This reaction produces a flash of yellow-green light with an emission peak around 560nm that can be detected by a luminometer (3). Firefly luciferase has become one of the more widely used reporter proteins and is an excellent tool for the study of gene expression, given that the amount of light emitted is directly proportional to luciferase activity (4).

The luciferase assay is fast and sensitive, differentiating itself from the CAT (chloramphenicol acetyltransferase) assay because it does not require a radioactive substrate.

References

1. Eun, H. (1996). Marker/Reporter enzymes. Enzymology Primer for Recombinant DNA Technology, 567-645. doi:10.1016/b978-012243740-3/50011-9

2. McNabb, D. S., Reed, R., & Marciniak, R. A. (2005). Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae. Eukaryotic Cell, 4(9), 1539-1549. doi:10.1128/ec.4.9.1539-1549.2005

3. Fraga, H. (2008). Firefly luminescence: A historical perspective and recent developments. Photochemical & Photobiological Sciences, 7(2), 146-158. doi:10.1039/b719181b

4. Younes, A., Lukyanenko, Y. O., Lyashkov, A. E., Lakatta, E. G., & Sollott, S. J. (2011). A bioluminescence method for direct measurement of phosphodiesterase activity. Analytical Biochemistry, 417(1), 36-40. doi:10.1016/j.ab.2011.05.036

Alternate Names

LuC, luciferin 4 monooxygenase, Luciferin 4-monooxygenase

Additional Luciferase Products

Product Documents for Luciferase Antibody (Luci 21 1-107) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Luciferase Antibody (Luci 21 1-107) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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